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sndB [2018-04-08 17:55:27]
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sndB [2018-04-08 17:55:27]

N-acetylcysteine deacetylase
locus
BSU39470 utilization of S-(2-succino)cysteine
pI
5.32
mw
41.44 kDa
protein length
380 aa Sequence Blast
gene length
1140 bp Sequence Blast
function
utilization and detoxofication of S-(2-succino)cysteine
product
N-acetylcysteine deacetylase
essential
no
synonyms
yxeP
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.4|Sulfur metabolism] → [category|SW 2.6.4.3|Conversion of S-(2-succino)cysteine to cysteine]
  • [category|SW 2|Metabolism] → [category|SW 2.7|Detoxification reactions]

    • Gene

    Coordinates
    4,056,870 → 4,058,012

    Phenotypes of a mutant

  • a ''[gene|0DE66A10D9475DC57A140561E8532D3EC038FD6F|sndA] [gene|C99AA3FDBB561BCED60915078DC746690C071753|sndB] [gene|D1A8D231369F0BF48639B61094B3222EC4605B79|sndC]'' triple mutant does not grow with N-acetyl cysteine and S-(2-succino)cysteine as the single sources of sulfur [Pubmed|23944997,29626092]

    • The protein

    Catalyzed reaction/ biological activity

  • N-acetylcysteine + oxaloacetate --> cysteine + acetate [Pubmed|29626092]

    • Protein family

  • peptidase M20 family (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|D6F1906CE8386F78F451D6B794F74DE4AD25AC9C|YkuR], [protein|0DE66A10D9475DC57A140561E8532D3EC038FD6F|SndA], [protein|AE824A62089631F129C3AA469B6ACA71DC13D2F8|AmhX]
  • [protein|D1A8D231369F0BF48639B61094B3222EC4605B79|SndC]:

    • Structure

  • [PDB|1YSJ]

    • Expression and Regulation

    Operons

    genes
    [gene|19E6EF9CCA31440EAC046F8D6CA98B6A08589D58|yxeK]-[gene|EBC2B9107AD50DD8F8174FC0BE4AD90DEE6A7CCA|snaB]-[gene|D96EEDABB73AC66E6AB772431819E7C767DC8B9A|yxeM]-[gene|FFD5F07480A0E5A74852C69C436EE289BA72FC7F|yxeN]-[gene|F8C800CF71D21A1F3265977C4F0EE191D0FFE36A|yxeO]-[gene|C99AA3FDBB561BCED60915078DC746690C071753|sndB]-[gene|7DDB283173267AA106DC277787AAAB4F4F9975F4|yxeQ]
    description
    [Pubmed|10746760]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|16513748], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|50930C56C27D22715620A350220E3C56ADB41020|CymR]: repression, [Pubmed|16513748], in [regulon|50930C56C27D22715620A350220E3C56ADB41020|CymR regulon]

    • regulation

  • repressed in the presence of cysteine ([protein|50930C56C27D22715620A350220E3C56ADB41020|CymR]) [Pubmed|16513748]

    • additional information

  • the amount of the mRNA is substantially decreased upon depletion of [gene|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [PubMed|21815947]
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    Biological materials

    Mutant

  • MGNA-B712 (yxeP::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1711 NBRP B. subtilis, Japan]
  • BKE39470 (Δ[gene|C99AA3FDBB561BCED60915078DC746690C071753|sndB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE39470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GTCGGCCATTGTTCTTCTCC, downstream forward: _UP4_GTTATTGTATTGGAGACCAT
  • BKK39470 (Δ[gene|C99AA3FDBB561BCED60915078DC746690C071753|sndB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK39470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GTCGGCCATTGTTCTTCTCC, downstream forward: _UP4_GTTATTGTATTGGAGACCAT

    • References

  • 10746760,23944997,16513748,29626092