DEAD-box RNA helicase, important for adaptation to low temperatures
product
DEAD-box RNA helicase
Genomic Context
categories
[category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.3|DEAD-box RNA helicases][category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.6|Ribosome assembly]Gene
Coordinates
2,594,184 → 2,595,500
Phenotypes of a mutant
poor growth at low temperatures (16 to 20°C) [Pubmed|23175651]The protein
Catalyzed reaction/ biological activity
RNA helicaserequired for [SW|ribosome] assembly [Pubmed|23175651]ATP + H2O --> ADP + H+ + phosphate (according to UniProt)Protein family
[SW|helicase family] (according to UniProt)[SW|DEAD-box RNA helicases] (according to UniProt)Paralogous protein(s)
[protein|E169F9711635457EA65FA71CCE9D7FF29DD8DF02|CshA], [protein|EACD0C84AA3CE41EF05EAA3D25AA1153293EB562|DeaD], [protein|0362D937D985846AEE4D0DEA57B62F915E8D35E5|YfmL] [SW|Domains]
[SW|Helicase ATP-binding domain] (aa 35-208) (according to UniProt)[SW|Helicase C-terminal domain] (aa 235-385) (according to UniProt)Structure
[PDB|5IVL] ([protein|E169F9711635457EA65FA71CCE9D7FF29DD8DF02|CshA], 80% coverage, 40% identity) [Pubmed|28238534][SW|Localization]
cytoplasma, colocalizes with the ribosomes [Pubmed|16352840]Expression and Regulation
Operons
genes
[gene|C83BD80DE0FD187D07B914F64696438ED5130107|cshB]-[gene|3C8BA04BA2BD0FEA28E99DA39BEB710CE3F5922D|nfo]
description
[pubmed|22383849]
view in new tabBiological materials
Mutant
MGNA-C489 ([gene|C83BD80DE0FD187D07B914F64696438ED5130107|cshB]::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2487 NBRP B. subtilis, Japan]GP1051 (Δ[gene|C83BD80DE0FD187D07B914F64696438ED5130107|cshB]::cat), available in [SW|Jörg Stülke]'s labBKE25140 (Δ[gene|C83BD80DE0FD187D07B914F64696438ED5130107|cshB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE25140 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTTCCCAGCTCCTTAA, downstream forward: _UP4_AAGAAAAGAAAGTAGGGGAABKK25140 (Δ[gene|C83BD80DE0FD187D07B914F64696438ED5130107|cshB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK25140 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTTCCCAGCTCCTTAA, downstream forward: _UP4_AAGAAAAGAAAGTAGGGGAAExpression vectors
for expression/ purification from ''B. subtilis'' with C-terminal Strep-tag, for [SW|SPINE], expression from the native chromomsomal site: GP1064 (spc), available in [SW|Jörg Stülke]'s labtwo-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab, [pubmed|20572937]FLAG-tag construct
GP1011 (spc, based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lablabs
[SW|Mohamed Marahiel], Marburg University, Germany [http://www.uni-marburg.de/fb15/fachgebiete/bio/marahiel?language_sync=1 homepage]References
16352840,12399512,20572937,23175651,28238534,30337909