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desK [Thu Dec 15 2016 14:44:03 GMT+0100 (CET)]
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desK [Thu Dec 15 2016 14:44:03 GMT+0100 (CET)]

two-component sensor kinase, regulation of cold shock expression of des
locus
BSU19190
pI
9.00
mw
42.00 kDa
function
regulation of cold shock expression of des
product
two-component sensor kinase
essential
no
synonyms
yocF
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.3|Lipid metabolism/ other]
  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.2|Protein kinases]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.2|Control of two-component response regulators] → [category|SW 3.4.2.2.1|Two-component sensor kinase]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.5|Cold stress proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]

    • [SW|Categories] containing this gene/protein

  • [SW|lipid metabolism/ other], [SW|protein modification], [SW|transcription factors and their control], [SW|cold stress proteins], [SW|membrane proteins], [SW|phosphoproteins]

    • Gene

    Coordinates on the chromosome (coding sequence)
    2,090,574 -> 2,091,686

    The protein

    Catalyzed reaction/ biological activity

  • autophosphorylation, phosphorylation of [protein|search|DesR]

    • [SW|Domains]

  • 5 transmembrane helices
  • cytoplasmatic C-terminal trail

    • Modification

  • autophosphorylation on a His residue

    • Effectors of protein activity

  • unsaturated fatty acids are negative effectors of the system

    • Structure

  • [PDB|3EHF]
  • [PDB|5IUJ]([SW|DesK]-[SW|DesR] complex in the phosphotransfer state with low Mg2 [20 mM]) [Pubmed|27938660]
  • [PDB|5IUK]([SW|DesK]-[SW|DesR] complex in the phosphotransfer state with high Mg2 [150 mM] and BeF3) [Pubmed|27938660]
  • [PDB|5IUM](phosphorylated wild type [SW|DesK]C) [Pubmed|27938660]
  • [PDB|5IUN]([SW|DesK]-[SW|DesR] complex in the phosphatase state) [Pubmed|27938660]

    • [SW|Localization]

  • membrane (transmembrane segments)

    • [SW|Interactions]

  • [protein|search|DesK]-[protein|search|DesR] [Pubmed|27938660]

    • Additional information

  • [protein|search|DesK] has the ability to sense changes in membrane fluidity [Pubmed|17087771]

    • Expression and Regulation

    Operon

  • ''[protein|search|desK]-[protein|search|desR]'' [Pubmed|11285232]

    • Regulation

  • induced by cold shock (12-fold) [Pubmed|12399512]

    • Biological materials

    Mutant

  • MGNA-A327 (yocF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/327 NBRP B. subtilis, Japan]

    • Labs working on this gene/protein

  • [SW|Diego de Mendoza], Universidad Nacional de Rosario, Argentine [http://www.ibr.gov.ar/eng/investigacion/demendoza.htm homepage]
  • [SW|Mohamed Marahiel], Marburg University, Germany [http://www.uni-marburg.de/fb15/fachgebiete/bio/marahiel?language_sync=1 homepage]

    • References

    Reviews

  • 20117042,17087771,24819366

    • Original publications

  • 10094672,14734164,12399512,20507988,19233289,11285232,19805278,15090506,12207704,20705470,23356219,24574048,24522108,25406381,26172072,27528507,27938660