tRNA:m1A22 methyl transferase
function
tRNA modification
product
tRNA:m1A22 methyl transferase
Genomic Context
categories
[category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.8|tRNA modification and maturation][category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]Gene
Coordinates
2,598,616 → 2,599,266
The protein
Catalyzed reaction/ biological activity
Catalyzes the S-adenosyl-L-methionine-dependent formation of N1-methyladenine at position 22 (m1A22) in tRNA [pubmed|18420655]adenosine22 in tRNA + S-adenosyl-L-methionine --> H+ + N1-methyladenosine22 in tRNA + S-adenosyl-L-homocysteine [pubmed|18420655]Protein family
TrmK family (single member, according to UniProt)[SW|Domains]
N-terminal Class I MTase domain [pubmed|30931478]C-terminal coiled-coil domain [pubmed|30931478]Modification
phosphorylation on Ser-48 [Pubmed|17218307][SW|Cofactors]
SAM [pubmed|30931478]Structure
[PDB|6Q56] [pubmed|30931478][SW|Localization]
cytoplasm (according to UniProt)Expression and Regulation
Operons
genes
[gene|C4EF534C1CA613038E1B7042A12EFAA6DA0B5B85|trmK]-[gene|DC60F31B3236C26C919AB7C574F13ABE98E976AF|yqfO]
description
[pubmed|22383849]view in new tabBiological materials
Mutant
BKE25180 (Δ[gene|C4EF534C1CA613038E1B7042A12EFAA6DA0B5B85|trmK]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE25180 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCACCGCTCCATTCGGTA, downstream forward: _UP4_GATCATGGCTAAAAGTGTAABKK25180 (Δ[gene|C4EF534C1CA613038E1B7042A12EFAA6DA0B5B85|trmK]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK25180 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCACCGCTCCATTCGGTA, downstream forward: _UP4_GATCATGGCTAAAAGTGTAAReferences
18420655,17218307,27098549,30931478 
