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pucF
allantoate amidohydrolase
function
purine utilization
product
allantoate amidohydrolase
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.1|Utilization of nucleotides]Gene
Coordinates
3,342,433 → 3,343,671
The protein
Catalyzed reaction/ biological activity
allantoate + 2 H+ + H2O --> (S)-2-ureidoglycine + CO2 + NH4+ (according to UniProt)Protein family
[SW|peptidase M20 family] (according to UniProt)Structure
[PDB|2IMO] (from ''Escherichia coli k12'', 47% identity, 63% similarity) [Pubmed|17362992]Expression and Regulation
Operons
genes
[gene|C314CE9D194E8894430368C5F69C0778D45A651C|pucF]-[gene|4BC4B3E0AB4779D1DD5EDF591361FC1094AF661F|pucG]
description
[Pubmed|12029039]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|12029039], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]regulatory mechanism
[protein|52C1601482C26400A524E880334BB801F832D6ED|PucR]: activation, [Pubmed|12029039], in [regulon|52C1601482C26400A524E880334BB801F832D6ED|PucR regulon]regulation
induced in the presence of purine nucleotides (inducer: allantoin) ([protein|search|PucR]) [Pubmed|12029039]view in new tabBiological materials
Mutant
MGNA-A943 (yurH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/943 NBRP B. subtilis, Japan]BKE32530 (Δ[gene|C314CE9D194E8894430368C5F69C0778D45A651C|pucF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE32530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTACTCCCCCCTCTGGC, downstream forward: _UP4_GCTTACTGATAAAGGAGGAABKK32530 (Δ[gene|C314CE9D194E8894430368C5F69C0778D45A651C|pucF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK32530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTACTCCCCCCTCTGGC, downstream forward: _UP4_GCTTACTGATAAAGGAGGAAReferences
