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malS [2019-08-08 13:58:48]
malate dehydrogenase (decarboxylating), forms a transhydrogenation cycle with [protein|search|YtsJ ]for balancing of NADPH
function
malate utilization
product
malate dehydrogenase (decarboxylating)
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.1|Utilization of organic acids]Gene
Coordinates
3,056,849 → 3,058,549
The protein
Catalyzed reaction/ biological activity
(S)-malate NAD = pyruvate CO2 NADH (according to Swiss-Prot) malate -→ pyruvateProtein family
[SW|malic enzymes family] (according to UniProt)Paralogous protein(s)
[protein|4F30A4412768E6313F57BD56C4EF5079F500E0C4|MaeA], [protein|45229A538D5124B60CE05DDC8699B9BC3D5AD9FF|YtsJ], [protein|160EBB7885D7C7FD30A6E35605A862C156E2DA80|MleA] Structure
[PDB|1LLQ] (from ''Ascaris suum'', 40% identity) [Pubmed|12033925]Expression and Regulation
Operons
genes
[gene|53259B300509E6B6DAF3ED106D1DDEDC99B5532B|ytnP]-[gene|BBBCD5F56779895189E21076AF165B901F654534|malS]
description
[Pubmed|22383849]
genes
[gene|BBBCD5F56779895189E21076AF165B901F654534|malS]
description
[Pubmed|9387221]
Biological materials
Mutant
GP1142 (Δ[gene|BBBCD5F56779895189E21076AF165B901F654534|malS]::spc) available in [SW|Jörg Stülke]'s labBKE29880 (Δ[gene|BBBCD5F56779895189E21076AF165B901F654534|malS]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29880 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACCCTGCCTCTTCCTTTCT, downstream forward: _UP4_TAATAAAAAAGAGCCGCTGCBKK29880 (Δ[gene|BBBCD5F56779895189E21076AF165B901F654534|malS]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29880 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACCCTGCCTCTTCCTTTCT, downstream forward: _UP4_TAATAAAAAAGAGCCGCTGCReferences
9387221,16788182,12949160,23136871,22740702,12033925 
