SubtiBank SubtiBank
glnH [2018-05-04 13:24:03]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

glnH [2018-05-04 13:24:03]

glutamine ABC transporter (binding protein)
locus
BSU27440
pI
8.55
mw
29.60 kDa
protein length
273 aa Sequence Blast
gene length
819 bp Sequence Blast
function
glutamine uptake
product
glutamine ABC transporter (binding protein)
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.1|Importers] → [category|SW 1.2.1.1.2|Uptake of amino acids]
  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.1|Biosynthesis/ acquisition of glutamate/ glutamine/ ammonium assimilation]
  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.1|Utilization of glutamine/ glutamate]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    2,803,108 → 2,803,929

    The protein

    Structure

  • [PDB|2V25] (from ''Campylobacter jejuni'', 37% identity) [Pubmed|17631313]

    • [SW|Localization]

  • attached to the membrane via [protein|6CE6AFDBE85974B45041DE924A09CC9F56ED79D8|GlnM]-[protein|CD32011A08697B2C121996A02F1BD7BE4F932778|GlnP] [Pubmed|10092453]

    • Expression and Regulation

    Operons

    genes
    [gene|468BEA9EAEA589D683DB79B2C689A5C6A22AB67F|glnQ]-[gene|BAB70547672C855AEB3A4D4624044340CF582848|glnH]-[gene|6CE6AFDBE85974B45041DE924A09CC9F56ED79D8|glnM]-[gene|CD32011A08697B2C121996A02F1BD7BE4F932778|glnP]
    description
    [Pubmed|12823818]

    sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|15699190], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]

    • regulatory mechanism

  • [protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA]: activation, [Pubmed|12823818], in [regulon|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA regulon]

    • regulation

  • expressed during sporulation ([protein|search|SigE]) [Pubmed|15699190]
    • view in new tab

    Biological materials

    Mutant

  • GP734 (spc), available in the [SW|Stülke] lab
  • BKE27440 (Δ[gene|BAB70547672C855AEB3A4D4624044340CF582848|glnH]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE27440 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACAAGCTCCCCCATTTC, downstream forward: _UP4_TAACAACCATCCCGGGATGA
  • BKK27440 (Δ[gene|BAB70547672C855AEB3A4D4624044340CF582848|glnH]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK27440 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACAAGCTCCCCCATTTC, downstream forward: _UP4_TAACAACCATCCCGGGATGA

    • References

  • 10092453,12823818,25755103,15699190,17631313,20023025