RNase III; required for the degradation of sense/antisense transcripts in Gram positive, low GC bacteria
function
cleaves both 5- and 3-sites of the small cytoplasmic RNA precursor (scr)
product
endoribonuclease III
Genomic Context
categories
[category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.4|RNases] → [category|SW 3.2.4.2|Endoribonucleases][category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.2|rRNA modification and maturation][category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.5|Protein secretion][category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]Gene
Coordinates
1,665,710 → 1,666,459
Phenotypes of a mutant
essential [Pubmed|23300471,12682299,11123676]the ''[gene|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|rnc]'' gene can be deleted in strains cured of the [SW|Skin element] and [[SPß prophage]] (or upon deletion of the toxin genes ''[gene|2A6EE9A3A5B32554E87E356B8BD4E2ED7975429A|txpA]'' and ''[gene|961DB64225CEF84D80982ADBDC9BBBCDF5049132|yonT]'') [Pubmed|23300471]The protein
Catalyzed reaction/ biological activity
Endonucleolytic cleavage of double-stranded RNA to 5'-phosphomonoester (according to Swiss-Prot)cleaves both 5'- and 3'-sites of the small cytoplasmic RNA precursor (''[gene|097C817A5A3E31F73F6ABC4AA95852A64E43A057|scr]'')degradation of ''[gene|2A6EE9A3A5B32554E87E356B8BD4E2ED7975429A|txpA]'' mRNA-[protein|search|FFCE5AD9E04E6D5F7970E89781C545E7DBDF45F0] RNA duplex (encoded on the [SW|Skin element]) [Pubmed|23300471]degradation of ''[gene|961DB64225CEF84D80982ADBDC9BBBCDF5049132|yonT]'' mRNA-''[gene|8832F99BF927D5A4D6CB265DB24D3AE46434A5CD|SR6]'' RNA duplex (encoded on the [[SP-beta prophage]]) [Pubmed|23300471]degradation of the ''[gene|6AC80B5916EEA851E9A7AF586341C58B7E69D8E7|roxS]''-''[gene|B729EFC0BF0D75765C2D2B0B4F397E0AEE11F1E7|ytdI]'' and ''[gene|6AC80B5916EEA851E9A7AF586341C58B7E69D8E7|roxS]-[gene|D72175937B3956C2BC500FC00CEC8EC57A0A76A8|sucC]'' RNA duplexes [Pubmed|25643072]Structure
[PDB|1O0W] (from ''Thermotoga maritima'', 41% identity)[SW|Localization]
cytoplasm (according to Swiss-Prot)Expression and Regulation
Operons
genes
[gene|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|rnc]-[gene|0136394A52759CEEDD8DE074ED74033C6FFB8435|smc]-[gene|D519765BF5261E48512CE86245BD7B733F188D4A|ftsY]
description
[Pubmed|11021934]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|11021934], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]regulatory mechanism
[regulon|stringent response|stringent response]: negative regulation, in [regulon|stringent response|stringent response]regulation
expressed during vegetative growth and during sporulation in the mother cell ([protein|search|SigK], [protein|search|GerE]) [Pubmed|11021934]view in new tabBiological materials
two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Stülke] labLabs working on this gene/protein
[SW|David Bechhofer], Mount Sinai School, New York, USA [http://www.mountsinai.org/Research/Centers Laboratories and Programs/Bechhofer Laboratory?citype=Physician&ciid=Bechhofer David H 1255565 Homepage][SW|Ciaran Condon], IBPC, Paris, France [http://www.ibpc.fr/UPR9073/condon/index_en.html Homepage]References
Reviews
17194582,16179796,8936814,16855311,23300472,25292357,25808661,25878039 Original publications
9393702,11123676,7527387,9677377,17576666,11021934,6409421,22123973,22412379,25643072,23300471,26940229,26883633 
