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narG [2019-06-19 12:23:19]
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narG [2019-06-19 12:23:19]

nitrate reductase (alpha subunit)
locus
BSU37280
pI
6.06
mw
138.86 kDa
protein length
1228 aa Sequence Blast
gene length
3687 bp Sequence Blast
function
nitrate respiration, nitrogen assimilation
product
nitrate reductase (alpha subunit)
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.1|Electron transport and ATP synthesis] → [category|SW 2.1.2|Respiration] → [category|SW 2.1.2.2|Anaerobic respiration]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    3,826,259 → 3,829,945

    The protein

    Catalyzed reaction/ biological activity

  • Nitrite + acceptor = nitrate + reduced acceptor (according to Swiss-Prot)

    • Protein family

  • prokaryotic molybdopterin-containing oxidoreductase family (according to Swiss-Prot)

    • [SW|Cofactors]

  • Fe-S cluster [Pubmed|11289299]
  • MoCo [Pubmed|11289299]

    • Structure

  • [PDB|1Q16] (the [protein|B992698A12EE9D17DCEF075F82396D5456D35A20|NarG]-[protein|6181C785427277C097B81D67A93213AEAF701FF4|NarH]-[protein|20060F4C1939FB9A10CB279745D75D0A16627EC6|NarI] complex of E. coli, 52% identity) [pubmed|12910261]

    • [SW|Localization]

  • cell membrane [Pubmed|23880299]

    • Expression and Regulation

    Operons

    genes
    [gene|B992698A12EE9D17DCEF075F82396D5456D35A20|narG]-[gene|6181C785427277C097B81D67A93213AEAF701FF4|narH]-[gene|5528AD62C1BF608D181C8960C88620B63B1E60A0|narJ]-[gene|20060F4C1939FB9A10CB279745D75D0A16627EC6|narI]
    description
    [Pubmed|8846791,16428414]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8846791,16428414], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|7165CC59CDAF64AAE2D591936860304A53BE5DF6|Fnr]: activation, [Pubmed|8846791,16428414], in [regulon|7165CC59CDAF64AAE2D591936860304A53BE5DF6|Fnr regulon]

    • regulation

  • expressed under anaerobic conditions ([protein|search|Fnr]) [Pubmed|8846791,16428414]
    • view in new tab

    Biological materials

    Mutant

  • BKE37280 (Δ[gene|B992698A12EE9D17DCEF075F82396D5456D35A20|narG]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE37280 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAGAGTTCACTCCTTCC, downstream forward: _UP4_CGCAAGCTGAAGGAGGTCGA
  • BKK37280 (Δ[gene|B992698A12EE9D17DCEF075F82396D5456D35A20|narG]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK37280 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAGAGTTCACTCCTTCC, downstream forward: _UP4_CGCAAGCTGAAGGAGGTCGA

    • References

    Reviews

  • 11289299

    • Original publications

  • 9352926,23880299,8846791,16428414,12910261