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dhbE [2017-11-16 13:43:37]
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dhbE [2017-11-16 13:43:37]

2,3-dihydroxybenzoate-AMP ligase (enterobactin synthetase component E)
locus
BSU31980
pI
5.68
mw
59.76 kDa
protein length
539 aa Sequence Blast
gene length
1617 bp Sequence Blast
function
biosynthesis of the siderophore bacillibactin
product
2,3-dihydroxybenzoate-AMP ligase (enterobactin synthetase component E)
essential
no
synonyms
entE
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.3|Acquisition of iron] → [category|SW 1.3.3.3|Acquisition of iron / Other]
  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.5|Iron metabolism] → [category|SW 2.6.5.1|Acquisition of iron / Other]

    • Gene

    Coordinates
    3,288,641 → 3,290,260

    The protein

    Protein family

  • ATP-dependent AMP-binding enzyme family (according to Swiss-Prot)

    • Structure

  • [PDB|1MDB] (complex with DHB-adenylate), [PDB|1MDF]

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|42C8D303AABFA2A9706295A0F90B0B49F854B0BF|dhbA]-[gene|0D4D200095AD4F9B30179B9AF500306EDA61F5D1|dhbC]-[gene|B864EC6D21AD5E524AC13AE1BE41F5CD7FC399C5|dhbE]-[gene|1346D57F906EE5BD36F7FBFA1E4884EEBC8585FA|dhbB]-[gene|95B75CFF126AF0AD845E62B4036DD3B6DAC8C7FA|dhbF]-[gene|DD647083C2E6968AD26A41C9F4EFBA942D75207C|ybdZ]
    description
    [Pubmed|8921902]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8550523], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|3DEAC421B4B173C6BBC700E57790751B7AFDF319|SigI]: sigma factor, [pubmed|29914988], in [regulon|3DEAC421B4B173C6BBC700E57790751B7AFDF319|SigI regulon]

    • regulatory mechanism

  • [protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur]: repression, [pubmed|12354229], in [regulon|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|20817675], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]
  • [protein|65E2F7197D46F5C884CC96018EE4F3EEE94FFA18|Kre]: repression, in [regulon|65E2F7197D46F5C884CC96018EE4F3EEE94FFA18|Kre regulon]

    • regulation

  • induced by iron starvation (second wave to allow iron scavenging from the environment) ([protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur]) [Pubmed|29133393,12354229]
  • expression of the operon is strongly induced at the beginning of [SW|biofilm formation] [pubmed|31113899]

    • additional information

  • the ''[gene|42C8D303AABFA2A9706295A0F90B0B49F854B0BF|dhbA]-[gene|0D4D200095AD4F9B30179B9AF500306EDA61F5D1|dhbC]-[gene|B864EC6D21AD5E524AC13AE1BE41F5CD7FC399C5|dhbE]-[gene|1346D57F906EE5BD36F7FBFA1E4884EEBC8585FA|dhbB]-[gene|95B75CFF126AF0AD845E62B4036DD3B6DAC8C7FA|dhbF]'' operon is strongly unregulated in a ''[SW|kre]'' mutant [Pubmed|26110430]
    • view in new tab

    Biological materials

    Mutant

  • BKE31980 (Δ[gene|B864EC6D21AD5E524AC13AE1BE41F5CD7FC399C5|dhbE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE31980 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTGTATCCCTCCAGCA, downstream forward: _UP4_TAAAACAACAATTTGAGAGG
  • BKK31980 (Δ[gene|B864EC6D21AD5E524AC13AE1BE41F5CD7FC399C5|dhbE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK31980 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTGTATCCCTCCAGCA, downstream forward: _UP4_TAAAACAACAATTTGAGAGG

    • Labs working on this gene/protein

  • [SW|Mohamed Marahiel], Marburg University, Germany [http://www.uni-marburg.de/fb15/fachgebiete/bio/marahiel?language_sync=1 homepage]

    • References

  • 12221282,11112781,11790741,12354229,8921902,8550523,20817675,21815947,24223955,26110430