SubtiBank SubtiBank
yfkF
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yfkF

similar to multidrug-efflux transporter
locus
BSU_07910
pI
8.91
mw
42.53 kDa
protein length
391 aa Sequence Blast
gene length
1176 bp Sequence Blast
function
unknown
product
unknown
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.12|Other transporters]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.14|Resistance against toxins/ antibiotics/ based on similarity]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    863,862 → 865,037

    The protein

    Protein family

  • [SW|major facilitator superfamily] (according to UniProt)

    • Structure

  • [PDB|3WDO] (from E. coli, corresponds to aa 233 ... 344, 26% identity) [pubmed|23950222]

    • [SW|Localization]

  • cell membrane (according to UniProt)

    • Expression and Regulation

    Operons

    genes
    [gene|B56B38A1CCC0A73FFD9F3F4C9942356E6D818D2D|yfkF]
    description
    [Pubmed|22383849]
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    Biological materials

    Mutant

  • MGNA-C347 (yfkF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2345 NBRP B. subtilis, Japan]
  • BKE07910 (Δ[gene|B56B38A1CCC0A73FFD9F3F4C9942356E6D818D2D|yfkF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE07910 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGTGTCAGTTCTCCTTA, downstream forward: _UP4_TAATCAAAAAGCCTCCCGAC
  • BKK07910 (Δ[gene|B56B38A1CCC0A73FFD9F3F4C9942356E6D818D2D|yfkF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK07910 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGTGTCAGTTCTCCTTA, downstream forward: _UP4_TAATCAAAAAGCCTCCCGAC

    • References

  • 26528249,23950222