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acoA [2018-08-01 11:59:05]
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acoA [2018-08-01 11:59:05]

acetoin dehydrogenase E1 component (TPP-dependent alpha subunit)
locus
BSU08060
pI
4.91
mw
35.91 kDa
protein length
333 aa Sequence Blast
gene length
999 bp Sequence Blast
function
acetoin utilization
product
acetoin dehydrogenase E1 component (TPP-dependent alpha subunit)
essential
no
synonyms
yfjK
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.2|Utilization of acetoin]

    • Gene

    Coordinates
    879,002 → 880,003

    The protein

    Paralogous protein(s)

  • [protein|953DE0F0B81894ECFF4C0693511AC238BF3D0C0A|PdhA], [protein|9F298088C0A9EB7FE140C935AFC9243C6D4DE8AE|BkdAA]

    • Structure

  • [PDB|6CFO] (human PDH, E1, 38% identity)

    • [SW|Localization]

  • cytoplasm (homogeneous) [Pubmed|16479537]

    • Expression and Regulation

    Operons

    genes
    [gene|B0A333BDFAE816B48B856A1DD91B73C41584A8E4|acoA]-[gene|5F953C3C91EB65A031D04A1D91F1CF501F8EFD09|acoB]-[gene|C4B6C3EE560C8BD353FEABB1A607C89C20DC8D34|acoC]-[gene|2ECF05AEE1549861EB3AE62139F0E9DEE0B0F632|acoL]
    description
    [pubmed|11274109]

    sigma factors

  • [protein|1118A21CC581B0470EC6E7C178F2523CDCA70F93|SigL]: sigma factor, [PubMed|11274109], in [regulon|1118A21CC581B0470EC6E7C178F2523CDCA70F93|SigL regulon]

    • regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|10666464], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]
  • [protein|706864AAF36684AD5E46F30E9EB76315AB412700|AcoR]: activation, (interaction with [protein|1118A21CC581B0470EC6E7C178F2523CDCA70F93|SigL]-containing [SW|RNA polymerase]) [ PubMed|11274109], in [regulon|706864AAF36684AD5E46F30E9EB76315AB412700|AcoR regulon]
  • [protein|7165CC59CDAF64AAE2D591936860304A53BE5DF6|Fnr]: indirect positive regulation, in [regulon|7165CC59CDAF64AAE2D591936860304A53BE5DF6|Fnr regulon]

    • regulation

  • induced by acetoin ([protein|706864AAF36684AD5E46F30E9EB76315AB412700|AcoR]) [http://www.ncbi.nlm.nih.gov/sites/entrez/11274109 PubMed]
  • expression of the operon is strongly induced during [SW|biofilm formation] [pubmed|31113899]

    • additional information

  • the mRNA is substantially stabilized upon depletion of [gene|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|21815947]
    • view in new tab

    additional information

  • the mRNA is very stable (> 15 min) [pubmed|12884008]

    • Biological materials

    Mutant

  • MGNA-C280 (acoA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2278 NBRP B. subtilis, Japan]
  • BKE08060 (Δ[gene|B0A333BDFAE816B48B856A1DD91B73C41584A8E4|acoA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE08060 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTGTGCGCCTCCTTCT, downstream forward: _UP4_TATGAAAAAGGAGGAATGTA
  • BKK08060 (Δ[gene|B0A333BDFAE816B48B856A1DD91B73C41584A8E4|acoA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK08060 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTGTGCGCCTCCTTCT, downstream forward: _UP4_TATGAAAAAGGAGGAATGTA

    • Labs working on this gene/protein

  • [SW|Michel Debarbouille], Pasteur Institute, Paris, France [http://www.pasteur.fr/ip/easysite/go/03b-00000m-0ob/recherche/departements-scientifiques/microbiologie/unites-et-groupes/unite-de-biologie-des-bacteries-pathogenes-a-gram-positif/les-membres-de-l-equipe Homepage]

    • References

  • 22900538,21815947,11274109,10368162,10666464,12884008,16479537,11274109,16428414