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cwlD [2019-06-17 12:07:16]
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cwlD [2019-06-17 12:07:16]

N-acetylmuramoyl-L-alanine amidase, spore cortex peptidoglycan synthesis
locus
BSU01530
pI
9.79
mw
26.86 kDa
protein length
237 aa Sequence Blast
gene length
714 bp Sequence Blast
function
spore cortex peptidoglycan synthesis
product
N-acetylmuramoyl-L-alanine amidase
essential
no
ec
3.5.1.28
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.5|Cell wall/ other]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]

    • Gene

    Coordinates
    156,612 → 157,325

    The protein

    Catalyzed reaction/ biological activity

  • formation of muramic acid delta-lacton in spore cortex peptidoglycan [Pubmed|14679227]

    • Protein family

  • N-acetylmuramoyl-L-alanine amidase 3 family (according to Swiss-Prot)

    • [SW|Domains]

  • contains an amidase_3 domain (like [protein|5CDF32B0E78F01C696364555E3F0BC8DF2AA2089|CwlC], [protein|6A21293823151C6980BF52B31A4B249A8440F2E1|LytC], [protein|0AD75864794E597AA9160969ADE6FE0C7ED07B9F|YqiI], [protein|BE34A9CE3AF99E8880FC24E34E5D79A1627260AE|YrvJ])

    • Structure

  • [PDB|4RN7] (from Clostridium difficile, 30% identity, aa 43 - 234)

    • [SW|Localization]

  • secreted (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|64786CC240BC208571AA3B3D28B5167FEF8CD428|ybaK]-[gene|B09FB626274B81F00A4FB42D188E089095343182|cwlD]
    description
    [Pubmed|7559346]

    sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|15699190], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]
  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG]: sigma factor, [Pubmed|15699190,7559346], in [regulon|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG regulon]

    • regulatory mechanism

  • [protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA]: repression, [Pubmed|16267290], in [regulon|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA regulon]

    • regulation

  • expressed during sporulation ([protein|search|SigG]) [Pubmed|7559346]
    • view in new tab

    genes
    [gene|B09FB626274B81F00A4FB42D188E089095343182|cwlD]
    description
    [Pubmed|7559346]

    regulation

  • expressed during sporulation ([protein|search|SigG], [protein|search|SigE]) [Pubmed|7559346]
    • view in new tab

    Biological materials

    Mutant

  • BKE01530 (Δ[gene|B09FB626274B81F00A4FB42D188E089095343182|cwlD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE01530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCCTTCCCCTCCCGCTT, downstream forward: _UP4_TAATGGAGGGTTTTCTTGTG
  • BKK01530 (Δ[gene|B09FB626274B81F00A4FB42D188E089095343182|cwlD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK01530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCCTTCCCCTCCCGCTT, downstream forward: _UP4_TAATGGAGGGTTTTCTTGTG

    • References

  • 12646705,10498740,7559346,14679227,16267290,22123250,24405365