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ppaC

inorganic pyrophosphatase

locus

BSU_40550

4.51

33.83 kDa

protein length

309 aa Sequence Blast

gene length

930 bp Sequence Blast

function

recovery of phosphate ions from pyrophosphate

product

inorganic pyrophosphatase

essential

yes

3.6.1.1

synonyms

yybQ

Outlinks

KEGG BsubCyc SubtiList UniProt Expression Browser

Genomic Context

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categories

[category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.3|Phosphate metabolism]

[category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

Gene

Coordinates

4,168,204 4,169,133

Phenotypes of a mutant

essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

diphosphate + H₂O --> H⁺ + 2 phosphate (according to UniProt)

Protein family

PPase class C family (single member, according to UniProt)

[SW|Domains]

[SW|DHH-DHHA1 domain]

Modification

S-cysteinylation after diamide stress (C158) [Pubmed|17611193]

Cys158 is S-bacillithiolated by NaOCl stress in B. subtilis and other Bacillus species [Pubmed|21749987] [Pubmed|21749987]

[SW|Cofactors]

Mn²⁺ [pubmed|17095506]

Structure

[PDB|2HAW] (complex with substrate analogue p-nitrophosphate), [PDB|1WPM] [pubmed|17095506]

[SW|Localization]

cytoplasm (according to Swiss-Prot)

additional information

belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

Expression and Regulation

Operons

genes

[gene|AE44787B3EB8EF5811B5C447BE2012EC36547B0A|ppaC]

description

[Pubmed|22383849]

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Biological materials

Mutant

MGNA-B837 (yybQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1836 NBRP B. subtilis, Japan]

References

22938038,21749987,15533045,17611193,9845334,9782505,11342544,9633597,21749987,15378759,22383849,25733611,17095506