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psdS [2019-06-06 11:26:14]
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psdS [2019-06-06 11:26:14]

two-component sensor kinase, control of [gene|464DD1855E6B3195EE242637612A16C73FF32FC0|psdA]-[gene|AC7F08DC1DF7DD2F9AFF84041D357B286B1244AC|psdB] in response to lipid II-binding lantibiotics, such as nisin and gallidermin
locus
BSU34710
pI
7.36
mw
40.02 kDa
protein length
356 aa Sequence Blast
gene length
1071 bp Sequence Blast
function
resistance against toxic peptides
product
two-component sensor kinase
essential
no
synonyms
yvcQ
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.2|Protein kinases]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.2|Control of two-component response regulators] → [category|SW 3.4.2.2.1|Two-component sensor kinase]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.13|Resistance against toxins/ antibiotics]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]

    • Gene

    Coordinates
    3,566,358 3,567,428

    The protein

    Catalyzed reaction/ biological activity

  • autophosphorylation, phosphorylation of [protein|985E7F25C809AA5DF372101E64960DF704EED53F|PsdR]

    • [SW|Domains]

  • two transmembrane segments, C-terminal histidine phosphotransferase domain

    • Modification

  • autophosphorylation on a His residue

    • Structure

  • [PDB|4I5S] (from Streptococcus mutans, the C-terminal histidine phosphotransferase domain, 29% identity) [pubmed|23468592]

    • [SW|Localization]

  • cell membrane (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|985E7F25C809AA5DF372101E64960DF704EED53F|psdR]-[gene|A9525886E911164BDFF0A55DA9F5FDD1C1B7A3B6|psdS]-[gene|464DD1855E6B3195EE242637612A16C73FF32FC0|psdA]-[gene|AC7F08DC1DF7DD2F9AFF84041D357B286B1244AC|psdB]
    description
    [Pubmed|21078927]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|21078927], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|985E7F25C809AA5DF372101E64960DF704EED53F|PsdR]: activation, [Pubmed|21078927], in [regulon|985E7F25C809AA5DF372101E64960DF704EED53F|PsdR regulon]

    • regulation

  • ''[protein|search|psdA]'': induced by nisin, gallidermin ([protein|search|PsdR]) [Pubmed|21078927]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-B636 (yvcQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1635 NBRP B. subtilis, Japan]
  • BKE34710 ([gene|A9525886E911164BDFF0A55DA9F5FDD1C1B7A3B6|psdS]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE34710 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TAAGCGGTCGATGAGATAAG, downstream forward: _UP4_TAAGATTGGGGAGCGGAATT
  • BKK34710 ([gene|A9525886E911164BDFF0A55DA9F5FDD1C1B7A3B6|psdS]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK34710 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TAAGCGGTCGATGAGATAAG, downstream forward: _UP4_TAAGATTGGGGAGCGGAATT

    • References

  • 10094672,18394148,14651641,21283517,27188294,21078927,23468592