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ponA [2018-02-16 12:46:15]
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ponA [2018-02-16 12:46:15]

class A penicillin-binding protein 1A/1B, contributes to cell elongation and [SW|cell division]
locus
BSU22320
pI
4.75
mw
99.36 kDa
protein length
914 aa Sequence Blast
gene length
2742 bp Sequence Blast
function
bifunctional glucosyltransferase/ transpeptidase
product
penicillin-binding protein 1A/1B
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.7|Penicillin-binding proteins]
  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.2|Cell shape]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    2,341,444 → 2,344,188

    Phenotypes of a mutant

  • prevents bulging of the cells when grown at low Mg(2 ) concentrations, suppresses the lethal effect of a ''[gene|A4C8719E06F774A6EB4D79757CC79CF89E453A54|mreB]'' mutation [Pubmed|19192185]
  • deletion of ''ponA'' restores growth and normal shape of a ''[gene|7150BABA5086D5E2EB8102E4901A216F43576282|glmR]'' mutant on gluconeogenic carbon sources [Pubmed|21320184]

    • The protein

    Structure

  • [PDB|3DWK] (aa 71 ... 656, 38% identity)

    • [SW|Localization]

  • membrane associated [Pubmed|18763711]
  • localizes to the spore septum during sporulation [Pubmed|15758244,15262952]
  • localizes to the vegetative septum [Pubmed|15262952], this depends on [protein|7150BABA5086D5E2EB8102E4901A216F43576282|GlmR] [Pubmed|21320184]

    • Expression and Regulation

    Operons

    genes
    [gene|ED1E2011C7E43A8B7E9FD9A471BC11B9DEA73177|recU]-[gene|A8EEC896B279CFDDE9C3A6F146A04EC822E33A4F|ponA]
    description
    [Pubmed|7814321]

    sigma factors

  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]: sigma factor, [Pubmed|18179421], in [regulon|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM regulon]
  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|22211522], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulation

  • constitutive during vegetative growth [Pubmed|15758244]
    • view in new tab

    Biological materials

    Mutant

  • BKE22320 (Δ[gene|A8EEC896B279CFDDE9C3A6F146A04EC822E33A4F|ponA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE22320 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAACATCTCAACCTTTCG, downstream forward: _UP4_TAAACAAAAAAGCCGTCACC
  • BKK22320 (Δ[gene|A8EEC896B279CFDDE9C3A6F146A04EC822E33A4F|ponA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK22320 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAACATCTCAACCTTTCG, downstream forward: _UP4_TAAACAAAAAAGCCGTCACC

    • GFP fusion

  • 2083 [gene|0467447B10EE7C1FDF86022AB6FBDBABFD32A9E5|trpC]2 [gene|A8EEC896B279CFDDE9C3A6F146A04EC822E33A4F|ponA]::pSG1492 (cat Pxyl-gfpa-[gene|A8EEC896B279CFDDE9C3A6F146A04EC822E33A4F|ponA]1-394) [pubmed|14731276], available in [SW|Dirk Jan Scheffers]' lab and in the [http://bgsc.org BGSC]

    • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jeff Errington] lab

    • Labs working on this gene/protein

  • [SW|Jeff Errington], Newcastle University, UK [http://www.ncl.ac.uk/camb/staff/profile/jeff.errington homepage]

    • References

    Reviews

  • 21371139

    • Original publications

  • 8606187,15262952,9721295,10322023,7814321,18363795,14731276,21320184,18763711,19192185,18179421,23531131,18363795