SubtiBank SubtiBank
ytoQ
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ytoQ

unknown
locus
BSU_29850
pI
5.77
mw
16.65 kDa
protein length
148 aa Sequence Blast
gene length
447 bp Sequence Blast
function
unknown
product
unknown
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 6|Groups of genes] → [category|SW 6.7|Proteins of unknown function]

    • Gene

    Coordinates
    3,054,746 3,055,192

    Expression and Regulation

    Operons

    genes
    [gene|A66C34183025AFAD7D935C7B7E107BEE94F9B362|ytoQ]
    description
    [pubmed|22383849]
    view in new tab

    Biological materials

    Mutant

  • MGNA-B532 (ytoQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1531 NBRP B. subtilis, Japan]
  • BKE29850 ([gene|A66C34183025AFAD7D935C7B7E107BEE94F9B362|ytoQ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATAAAAATCCCTCCTT, downstream forward: _UP4_TAAGAAAAAAGCTTGTCGAT
  • BKK29850 ([gene|A66C34183025AFAD7D935C7B7E107BEE94F9B362|ytoQ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATAAAAATCCCTCCTT, downstream forward: _UP4_TAAGAAAAAAGCTTGTCGAT
  • BP1101 ([gene|A66C34183025AFAD7D935C7B7E107BEE94F9B362|ytoQ]::kan trp+) available at [SW|Jörg Stülke]'s and [SW|Fabian Commichau]'s labs

    • Expression vectors

  • IPTG inducible expression of Strep-ytoQ in E. coli: pBP641 (in [SW|pGP172]) and pBP642 (in [SW|pGP574]), available in [SW|Fabian Commichau]'s lab
  • Constitutive expression of ytoQ in B. subtilis: pBP639 (in [SW|pBQ200]), available in [SW|Fabian Commichau]'s lab, [Pubmed|29027347]
  • pBP770: expression of Strep-''ytoQ'' by [SW|pGP380]in ''B. subtilis'' suitable for [SW|SPINE], available in [SW|Jörg Stülke's] and [SW|Fabian Commichau]'s labs
  • pBP779: expression of ''ytoQ''-Strep by [SW|pGP382] in ''B. subtilis'' suitable for [SW|SPINE], available in [SW|Jörg Stülke]'s and [SW|Fabian Commichau]'s labs

    • lacZ fusion

  • pBP638 (in [SW|pAC7]), available in [SW|Fabian Commichau]'s lab [Pubmed|29027347]

    • labs

  • [SW|Fabian Commichau], Göttingen, Germany [http://www.uni-goettingen.de/de/413008.html homepage]

    • References

    Research papers

  • 11918677,29027347