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bcd [Tue Dec 13 2016 11:16:34 GMT+0100 (CET)]
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bcd [Tue Dec 13 2016 11:16:34 GMT+0100 (CET)]

valine dehydrogenase, isoleucine dehydrogenase, L-leucine dehydrogenase
locus
BSU24080
pI
4.94
mw
39.00 kDa
function
utilization of branched-chain keto acids
product
valine dehydrogenase, isoleucine dehydrogenase,
L-leucine dehydrogenase
essential
no
ec
1.4.1.9
synonyms
bkd, yqiT
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.9|Utilization of branched-chain amino acids]

    • [SW|Categories] containing this gene/protein

  • [SW|utilization of amino acids]

    • This gene is a member of the following [SW|regulons]

  • [SW|BkdR regulon], [SW|CodY regulon], [SW|SigL regulon]

    • Gene

    Coordinates on the chromosome (coding sequence)
    2,502,659 -> 2,503,753

    The protein

    Catalyzed reaction/ biological activity

  • L-leucine + H2O + NAD+ = 4-methyl-2-oxopentanoate + NH3 + NADH (according to Swiss-Prot)

    • Protein family

  • Glu/Leu/Phe/Val dehydrogenases family (according to Swiss-Prot)

    • Structure

  • [PDB|1LEH] (from '' Lysinibacillus sphaericus'', 71% identity, 82% similarity) [Pubmed|8591046]

    • Expression and Regulation

    Operon

  • ''[protein|search|ptb]-[protein|search|bcd]-[protein|search|buk]-[protein|search|lpdV]-[SW|bkdAA]-[SW|bkdAB]-[protein|search|bkdB]'' [Pubmed|12823818]

    • [SW|Sigma factor]

  • [protein|search|SigL] [Pubmed|10094682]

    • Regulation

  • induced in the presence of isoleucine or valine ([protein|search|BkdR]) [Pubmed|10094682]

    • Regulatory mechanism

  • [protein|search|CodY]: indirect regulation [Pubmed|24843172]
  • [protein|search|BkdR]: transcription activation [Pubmed|10094682]

    • Biological materials

    Mutant

  • a ''bcd'' mutant and a ''[protein|search|bcd] [protein|search|ybgE] [protein|search|ywaA]'' triple mutant are available in [SW|Linc Sonenshein]'s lab

    • References

    Reviews

  • 7705101

    • Original publications

  • 12427936,15241682,10094682,12823818,24843172