cell shape-determining protein, forms filaments, the polymers control/restrict the mobility of the cell wall elongation enzyme complex, required for LytE activity
function
cell shape determination
product
cell shape-determining protein
Genomic Context
categories
[category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.2|Cell shape][category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.7|Membrane dynamics][category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)][category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes][category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]Gene
Coordinates
2,860,735 → 2,861,748
Phenotypes of a mutant
essential [Pubmed|12682299]the mutation can be suppressed by inactivation of ''[gene|A8EEC896B279CFDDE9C3A6F146A04EC822E33A4F|ponA]'', ''[gene|14ED1AF5038F43F3B151FCBABE6CFC5A2DA3AA6E|ptsI]'', ''[gene|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]'' [Pubmed|19192185], by overexpression of [protein|7150BABA5086D5E2EB8102E4901A216F43576282|GlmR] [Pubmed|21320184], or by addition of 5 mM magnesium to the growth medium [Pubmed|15752190]The protein
Catalyzed reaction/ biological activity
forms straight filaments in a heterologous system [Pubmed|21091501]polymerizes in the presence of millimolar divalent cations, binds and hydrolyzes GTP and ATP [Pubmed|19117023]involved in the organization of ϕ29 DNA replication machinery in peripheral helix-like structures [Pubmed|22420858]required for [protein|321F248C22D7283C0F3323F1F4069E36F8D7FE6C|LytE] activity [Pubmed|23869552]Protein family
ftsA/mreB family (according to Swiss-Prot)Paralogous protein(s)
[protein|6ED386D49F973C1F6B1076974F54275DD583C9D3|Mbl], [protein|40829C3632E9DCEB9E1C14A674B4B217F504CFE0|MreBH]Modification
acetylated at Lys-240, this is important for restricting cell wall growth and cell diameter [Pubmed|27376153]Structure
[PDB|1JCF] (from ''Thermotoga maritima'', 57% identity) [Pubmed|11544518][SW|Localization]
during logarithmic growth, [protein|A4C8719E06F774A6EB4D79757CC79CF89E453A54|MreB] forms discrete patches that move processively along peripheral tracks perpendicular to the cell axis [Pubmed|21636744]forms transverse bands as cells enter the stationary phase [Pubmed|21636744]forms antiparallel double filaments [Pubmed|24843005]close to the inner surface of the cytoplasmic membrane [Pubmed|16950129]reports on helical structures formed by MreB [Pubmed|16950129,20566861] seem to be misinterpretation of data [Pubmed|21636744]normal localization depends on the presence of glucolipids, [protein|A4C8719E06F774A6EB4D79757CC79CF89E453A54|MreB] forms irregular clusters in an ''[gene|7A606B8E952AE8CA4F9A62008BA4B156725BB5B5|ugtP]'' mutant [Pubmed|22362028]during stationary phase: delocalized in the cytoplasm [Pubmed|26091431]during development of [SW|genetic competence]: co-localizes with polar clusters of the late competence protein [protein|41DFEF8A6BC7E34A6681C26D92F2B08DC120A957|ComGA] ([protein|41DFEF8A6BC7E34A6681C26D92F2B08DC120A957|ComGA] sequesters [protein|A4C8719E06F774A6EB4D79757CC79CF89E453A54|MreB]) [Pubmed|26091431]Expression and Regulation
Operons
genes
[gene|A4C8719E06F774A6EB4D79757CC79CF89E453A54|mreB]-[gene|6A14D0C7FD94A838BAB3B74AA100EA678F1E4E34|mreC]-[gene|231C30963AFB863424C087CB722446D319976D65|mreD]-[gene|8C94C9598A823A8405B3E1FA0124E21D90845B8E|minC]-[gene|37DAD42A391E2FC506225EAF91B8F21629A401DF|minD]
description
[Pubmed|8459776]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, promoter p1, upstream of [protein|49D996C9AED44A820A214C3DF60AB1B6E1508DEA|Maf] [Pubmed|26091431], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon][protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]: sigma factor, promoter p2, within [protein|49D996C9AED44A820A214C3DF60AB1B6E1508DEA|Maf] [Pubmed|18179421], in [regulon|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM regulon][protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH]: sigma factor, promoter p4, upstream of [protein|8C94C9598A823A8405B3E1FA0124E21D90845B8E|MinC] [Pubmed|8459776], in [regulon|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH regulon]regulatory mechanism
[protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|ComK]: activation, [Pubmed|26091431], in [regulon|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|ComK regulon]regulation
constitutively expressed [Pubmed|23701187]view in new tabgenes
[gene|49D996C9AED44A820A214C3DF60AB1B6E1508DEA|maf]-[gene|2BD11D71AA6D9EFAD8A209C8813CC8B059180C84|radC]-[gene|A4C8719E06F774A6EB4D79757CC79CF89E453A54|mreB]-[gene|6A14D0C7FD94A838BAB3B74AA100EA678F1E4E34|mreC]-[gene|231C30963AFB863424C087CB722446D319976D65|mreD]-[gene|8C94C9598A823A8405B3E1FA0124E21D90845B8E|minC]-[gene|37DAD42A391E2FC506225EAF91B8F21629A401DF|minD]
description
[Pubmed|22383849]
sigma factors
[protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]: sigma factor, promoter p2 within [protein|49D996C9AED44A820A214C3DF60AB1B6E1508DEA|Maf] [Pubmed|18179421], in [regulon|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM regulon][protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, promoter p1, upstream of [protein|49D996C9AED44A820A214C3DF60AB1B6E1508DEA|Maf] [Pubmed|26091431], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]regulatory mechanism
[protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|ComK]: activation, [Pubmed|11948146,11918817,21564336], in [regulon|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|ComK regulon]regulation
constitutively expressed [Pubmed|23701187]view in new tabBiological materials
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in the labs of [SW|Jeff Errington] and [SW|Boris Görke]Antibody
available in the [SW|Jeff Errington] and [SW|Peter Graumann] labsLabs working on this gene/protein
[SW|Jeff Errington], Newcastle University, UK [http://www.ncl.ac.uk/camb/staff/profile/jeff.errington homepage][SW|Peter Graumann], Freiburg University, Germany [http://www.biologie.uni-freiburg.de/data/bio2/graumann/index.htm homepage]References
Reviews
17506674,17158703,15037301,21725336,20825347,21047262,22166997,22154164,22652894,21371139,25578957,25957405 Localization
17064365,20566861,21636744,21636745,22069484,23783036,24010660,24843005,25402772,25676310 Other original publications
15752190,11290328,12809607,1400224,7836311,16950129,19192185,19117023,19643765,19654094,19659933,8459776,11544518,20133608,21091501,21320184,18179421,21964069,22343529,22362028,22420858,21926231,23879732,23869552,24261876,24603761,26091431,17880425,20233306,27215790,27376153,28500523 
