SubtiBank SubtiBank
yrhE
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yrhE

putative formate dehydrogenase
locus
BSU_27220
pI
5.16
mw
108.57 kDa
protein length
980 aa Sequence Blast
gene length
2943 bp Sequence Blast
function
unknown
product
unknown
essential
no
ec
1.2.1.2
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 6|Groups of genes] → [category|SW 6.7|Proteins of unknown function]

    • Gene

    Coordinates
    2,781,209 2,784,151

    The protein

    Catalyzed reaction/ biological activity

  • formate + NAD+ --> CO2 + NADH (according to UniProt)

    • Protein family

  • C-terminal part: [SW|prokaryotic molybdopterin-containing oxidoreductase family] (according to UniProt)

    • Paralogous protein(s)

  • [protein|E54F39E72DC4881E886159607C2B424A41DE0D01|YjgC]

    • [SW|Domains]

  • 2Fe-2S ferredoxin-type domain (aa 5-81) (according to UniProt)
  • 4Fe-4S His(Cys)3-ligated-type domain (aa 81-121) (according to UniProt)
  • 2 [SW|4Fe-4S ferredoxin-type domain]s (aa 144-171, aa 187-216) (according to UniProt)
  • [SW|4Fe-4S Mo/W bis-MGD-type domain] (aa 263-319) (according to UniProt)

    • Modification

  • Fe-S cluster [pubmed|29292548]

    • [SW|Cofactors]

  • contains an iron-sulfur cluster

    • Structure

  • [PDB|2FUG] (from Thermus thermophilus, 23% identity) [pubmed|16469879]

    • Additional information

  • The gene is annotated in KEGG as an ortholog of formate dehydrogenase EC 1.2.1.2. In Swiss-Prot the protein is named formate dehydrogenase chain A. In MetaCyc the protein is characterized as similar to formate dehydrogenase. No literature/experimental evidence supporting the annotation is available. [Pubmed|19935659]

    • Expression and Regulation

    Operons

    genes
    [gene|A4304213CFC152B601B2EED2B9B3A0A3AB4E6A81|yrhE]-[gene|364BC3B51D8DF152C2DF31046D4F80595C75884B|yrhD]
    description
    [Pubmed|21815947]

    additional information

  • term-seq has identified a potential novel regulatory RNA element including an intrinsic transcription terminator upstream of ''yrhE'' [Pubmed|27120414]
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    Biological materials

    Mutant

  • MGNA-A160 (yrhE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/160 NBRP B. subtilis, Japan]
  • BKE27220 ([gene|A4304213CFC152B601B2EED2B9B3A0A3AB4E6A81|yrhE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE27220 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTCATATTCCCCTCCA, downstream forward: _UP4_GATTAAACGATAGGAGGAAA
  • BKK27220 ([gene|A4304213CFC152B601B2EED2B9B3A0A3AB4E6A81|yrhE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK27220 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTCATATTCCCCTCCA, downstream forward: _UP4_GATTAAACGATAGGAGGAAA

    • References

  • 21815947,27120414,19935659,16469879