SubtiBank SubtiBank
ykuJ [2019-02-19 20:12:25]
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ykuJ [2019-02-19 20:12:25]

unknown
locus
BSU14100
pI
4.34
mw
9.11 kDa
protein length
79 aa Sequence Blast
gene length
240 bp Sequence Blast
function
unknown
product
unknown
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 6|Groups of genes] → [category|SW 6.7|Proteins of unknown function]

    • Gene

    Coordinates
    1,484,117 1,484,356

    The protein

    Structure

  • [PDB|2FFG]

    • Expression and Regulation

    Operons

    genes
    [gene|A26502ADAC214D45A8F7F394587B0BDFBE7AF28E|ykuJ]-[gene|7FD784C885605F7FD27898BC9E83C28F21BE0E9D|ykuK]-[gene|899FF5BEE5D3F01778A0175E293EDBBDEB25717D|abbA]-[gene|90AFF93D6E7BC993B2773C12EE400C80A87A4831|darB]-[gene|FE15A41A58A8177280817CA3825764C39185021A|ccpC]
    description
    [Pubmed|16395550]

    regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: activation, by [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]~P [Pubmed|18840696,14651647], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|16395550,12850135], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

    • regulation

  • expressed under conditions that trigger sporulation ([SW|Spo0A]) [Pubmed|14651647]
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    Biological materials

    Mutant

  • MGNA-A771 (ykuJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/771 NBRP B. subtilis, Japan]
  • GP214 (deletion of ''[gene|A26502ADAC214D45A8F7F394587B0BDFBE7AF28E|ykuJ]-[gene|7FD784C885605F7FD27898BC9E83C28F21BE0E9D|ykuK]-[gene|899FF5BEE5D3F01778A0175E293EDBBDEB25717D|abbA]-[gene|90AFF93D6E7BC993B2773C12EE400C80A87A4831|darB]'', replaced by ''aphA3''), available in the [SW|Stlke] lab
  • BKE14100 ([gene|A26502ADAC214D45A8F7F394587B0BDFBE7AF28E|ykuJ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE14100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTAAAAAAAGACTCCT, downstream forward: _UP4_TAAGATTCCTGAAATAGGGG
  • BKK14100 ([gene|A26502ADAC214D45A8F7F394587B0BDFBE7AF28E|ykuJ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK14100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTAAAAAAAGACTCCT, downstream forward: _UP4_TAAGATTCCTGAAATAGGGG

    • lacZ fusion

  • pGP710 (in [protein|search|pAC5]), available in [SW|Stlke] lab, pGP714 (in [protein|search|pAC6]), available in [SW|Stlke] lab

    • References

  • 16395550