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bmrD
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bmrD

multidrug [SW|ABC transporter ](ATP-binding protein), also involved in the signalling pathway to activate [protein|search|KinA ]at the onset of [SW|sporulation]
locus
BSU_09720
pI
7.08
mw
76.12 kDa
protein length
673 aa Sequence Blast
gene length
2022 bp Sequence Blast
function
multiple antibiotic resistance
product
multidrug [SW|ABC transporter ](ATP-binding protein)
essential
no
synonyms
yheH
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.2|Exporters] → [category|SW 1.2.1.2.1|Efflux of antibiotics]
  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.3|Regulatory ABC transporters]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.13|Resistance against toxins/ antibiotics]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    1,047,072 1,049,093

    The protein

    Protein family

  • [SW|ABC transporter superfamily] (according to UniProt)

    • Paralogous protein(s)

  • [protein|0EB4D3E1B9AF39ECCBD79EB559FAE5BB0EE4C156|YgaD]

    • [SW|Domains]

  • has both a membrane-spanning and an ATP-binding domain [Pubmed|10092453]
  • [SW|ABC transmembrane type-1 domain] (aa 18-398) (according to UniProt)
  • [SW|ABC transporter domain] (aa 430-664) (according to UniProt)

    • Structure

  • [PDB|5MKK] (TmrAB complex from Thermus thermophilus, 38% identity) [pubmed|28069938]

    • [SW|Localization]

  • cell membrane [Pubmed|10092453]

    • Expression and Regulation

    Operons

    genes
    [gene|E9956A958C66C4114EB974EA6F9BF30CD951034A|bmrB]-[gene|67C0C9EA17EF953554D3D57CD0D285246F50C1AD|bmrC]-[gene|9B2766D2F8892AA17DB480216570C81C33355C3B|bmrD]
    description
    [Pubmed|25217586]

    regulatory mechanism

  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|20817675,25217586], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]
  • [protein|E9956A958C66C4114EB974EA6F9BF30CD951034A|BmrB]: attenuation, [Pubmed|25217586], in [regulon|E9956A958C66C4114EB974EA6F9BF30CD951034A|BmrB regulon]

    • regulation

  • ''[protein|search|bmrB]-[protein|search|bmrC]-[protein|search|bmrD]'' expression only occurs during the late-exponential and stationary growth stages ([protein|search|AbrB]) [Pubmed|25217586]
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    Biological materials

    Mutant

  • MGNA-B491 (yheH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1490 NBRP B. subtilis, Japan]
  • BKE09720 ([gene|9B2766D2F8892AA17DB480216570C81C33355C3B|bmrD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09720 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TCTCCATAACGTTTTTCCTA, downstream forward: _UP4_TAACGCTCAAAAACCCAAAA
  • BKK09720 ([gene|9B2766D2F8892AA17DB480216570C81C33355C3B|bmrD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09720 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TCTCCATAACGTTTTTCCTA, downstream forward: _UP4_TAACGCTCAAAAACCCAAAA

    • References

  • 19167342,10092453,16487324,25217586,21602923,29507890,28069938