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khtU [2019-05-02 17:56:31]
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khtU [2019-05-02 17:56:31]

K+/H+ antiporter for K+ efflux
locus
BSU09850
pI
9.29
mw
43.79 kDa
protein length
405 aa Sequence Blast
gene length
1218 bp Sequence Blast
function
potassium ion efflux
product
K+/H+ antiporter for K+ efflux
essential
no
synonyms
yhaU
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.5|Metal ion transporter]
  • [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.1|Metal ion homeostasis (K, Na, Ca, Mg)] → [category|SW 1.3.1.3|Potassium uptake/ export]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    1,059,203 1,060,420

    Phenotypes of a mutant

  • increased sensitivity to methylglyoxal [Pubmed|24330391]

    • The protein

    Structure

  • [PDB|4BWZ] (sodium proton antiporter NapA from Thermus thermophilus, 26% identity) [pubmed|23995679]

    • [SW|Localization]

  • cell membrane, integral membrane protein (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|A54E8E38AEF596D09B4BEB64D9F539A8AD34E73D|khtS]-[gene|D8A8FBC05B9665F72A88685D2B33822C2BB84C62|khtT]-[gene|9A54C82323BA9220CAA6041E3745FAB9E222FE78|khtU]
    description
    [Pubmed|22383849]
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    Biological materials

    Mutant

  • MGNA-A682 (yhaU::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/682 NBRP B. subtilis, Japan]
  • GP2078 ([gene|A54E8E38AEF596D09B4BEB64D9F539A8AD34E73D|khtS]-[gene|D8A8FBC05B9665F72A88685D2B33822C2BB84C62|khtT]-[gene|9A54C82323BA9220CAA6041E3745FAB9E222FE78|khtU]::''aphA3''), available in [SW|Jörg Stülke]'s lab
  • BKE09850 ([gene|9A54C82323BA9220CAA6041E3745FAB9E222FE78|khtU]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AAATACAAGATGGTCCATCG, downstream forward: _UP4_GGATAAAAAAAGCAGCACTG
  • BKK09850 ([gene|9A54C82323BA9220CAA6041E3745FAB9E222FE78|khtU]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AAATACAAGATGGTCCATCG, downstream forward: _UP4_GGATAAAAAAAGCAGCACTG

    • FLAG-tag construct

  • GP2432 ''khtU-3xFLAG spc'' (based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab

    • References

    Reviews

  • 27935846

    • Original publications

  • 24330391,14987767,17679694,22383849,23995679