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ykkE
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ykkE

formyltetrahydrofolate deformylase
locus
BSU_13110
pI
5.95
mw
34.34 kDa
protein length
300 aa Sequence Blast
gene length
903 bp Sequence Blast
function
purine nucleotide synthesis
product
formyltetrahydrofolate deformylase
essential
no
ec
3.5.1.10
synonyms
purU
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.2|Biosynthesis/ acquisition of nucleotides] → [category|SW 2.5.2.1|Biosynthesis/ acquisition of purine nucleotides]

    • Gene

    Coordinates
    1,377,243 1,378,145

    The protein

    Catalyzed reaction/ biological activity

  • (6S)-10-formyltetrahydrofolate + H2O --> (6S)-5,6,7,8-tetrahydrofolate + formate + H+ (according to UniProt)

    • Protein family

  • PurU family (single member, according to UniProt)

    • Paralogous protein(s)

  • [protein|7B30F27D6C459437967A64467E52961F4A9E0F2C|PurN]

    • [SW|Domains]

  • [SW|ACT domain] (aa 21-102) (according to UniProt)

    • Structure

  • [PDB|3W7B] (from ''Thermus thermophilus'', 58% identity) [Pubmed|24108189]

    • Expression and Regulation

    Operons

    genes
    [gene|9A399CAA672C3DF90531A7A7168916DE86EFA365|ykkE]
    description
    [Pubmed|22383849]
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    Biological materials

    Mutant

  • MGNA-B310 (ykkE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1309 NBRP B. subtilis, Japan]
  • BKE13110 ([gene|9A399CAA672C3DF90531A7A7168916DE86EFA365|ykkE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE13110 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAATAATCCCTCTTATC, downstream forward: _UP4_TAGACTGCAAGAGGCCCGCG
  • BKK13110 ([gene|9A399CAA672C3DF90531A7A7168916DE86EFA365|ykkE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK13110 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAATAATCCCTCTTATC, downstream forward: _UP4_TAGACTGCAAGAGGCCCGCG

    • References

  • 24108189,22383849