SubtiBank SubtiBank
nucA [2017-11-28 10:27:17]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

nucA [2017-11-28 10:27:17]

membrane-associated nuclease, catalyzes DNA cleavage during transformation
locus
BSU03430
pI
4.68
mw
16.12 kDa
protein length
147 aa Sequence Blast
gene length
441 bp Sequence Blast
function
genetic transformation, DNA uptake
product
membrane-associated nuclease
essential
no
synonyms
comI
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.7|Genetic competence]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.3|Genetic competence]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    372,154 → 372,597

    The protein

    [SW|Localization]

  • integral membrane protein [Pubmed|11359569]

    • Expression and Regulation

    Operons

    genes
    [gene|99581CC6AFFA2D1392748B4D9C99DEF8B7601F75|nucA]-[gene|3A54C644DD1CCA5C08CB9C5CD648D44889E68D23|nin]
    description
    [Pubmed|11359569]

    regulatory mechanism

  • [protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|ComK]: activation, [Pubmed|7746143], in [regulon|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|ComK regulon]
    • view in new tab

    Biological materials

    Mutant

  • BKE03430 (Δ[gene|99581CC6AFFA2D1392748B4D9C99DEF8B7601F75|nucA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE03430 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GATGTTCACCTCCCGCTTTT, downstream forward: _UP4_TAAACAGTACATAGAGGAGG
  • BKK03430 (Δ[gene|99581CC6AFFA2D1392748B4D9C99DEF8B7601F75|nucA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK03430 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GATGTTCACCTCCCGCTTTT, downstream forward: _UP4_TAAACAGTACATAGAGGAGG

    • References

  • 11814663,11359569,7746143