two-component sensor kinase of the YvrG-YvrHb two-component system
function
regulation cell surface maintenance
product
two-component sensor histidine kinase
Genomic Context
categories
[category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.2|Protein kinases][category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.2|Control of two-component response regulators] → [category|SW 3.4.2.2.1|Two-component sensor kinase][category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins][category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]Gene
Coordinates
3,406,614 3,408,356
Phenotypes of a mutant
unusual autolysis and higher susceptibility to cell envelope antibiotics (bacitracin, aztreonam, cefepime, fosfomycin) [Pubmed|16306698]The protein
Catalyzed reaction/ biological activity
autophosphorylation, phosphorylation of the [protein|7098319D8E88FDC2A629A3F4A21507FD7A649385|YvrHb] response regulator [Pubmed|16306698]ATP + protein L-histidine --> ADP + protein N-phospho-L-histidine (according to UniProt)Paralogous protein(s)
[protein|9E8A6AB3920BFBFDAAD1F2E4F333A32354ABB25B|YkoH], [protein|1582E81F7DA85F38D6732D341ABD0F052587F25A|KinE], [protein|4EE48E4931F662E51586DB6D01E91C688329222D|CssS], [protein|52BB660B0CAB36C74F1D47963235846B45AF78AB|YclK] [SW|Domains]
two transmembrane segments[SW|Histidine kinase domain] (aa 363-580) (according to UniProt)Modification
autophosphorylation on a His residue[SW|Localization]
cell membrane (according to UniProt)Expression and Regulation
Operons
genes
[gene|7098319D8E88FDC2A629A3F4A21507FD7A649385|yvrHb]-[gene|96E4470EF4B558F4EC318FEBF58B779BA50DB7E2|yvrG]-[gene|0F65F634368E935585FC015BA64085F3A2244F9B|yvrE]
description
[Pubmed|22383849]
view in new tabBiological materials
Mutant
MGNA-B049 (yvrG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1048 NBRP B. subtilis, Japan]BKE33210 ([gene|96E4470EF4B558F4EC318FEBF58B779BA50DB7E2|yvrG]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE33210 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CCTCATGACCGCTTGCCTTC, downstream forward: _UP4_TAAACATCAAGTGGTTTGGABKK33210 ([gene|96E4470EF4B558F4EC318FEBF58B779BA50DB7E2|yvrG]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK33210 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CCTCATGACCGCTTGCCTTC, downstream forward: _UP4_TAAACATCAAGTGGTTTGGAReferences
10094672,18820022,16306698