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thiS
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thiS

sulfur carrier protein, hydroxyethylthiazole phosphate biosynthesis
locus
BSU_11680
pI
5.50
mw
7.49 kDa
protein length
66 aa Sequence Blast
gene length
201 bp Sequence Blast
function
biosynthesis of thiamine
product
sulfur carrier protein
essential
no
synonyms
yjbS
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.3|Biosynthesis/ acquisition of thiamine]

    • Gene

    Coordinates
    1,244,844 1,245,044

    The protein

    Protein family

  • sulfur carrier protein ThiS family (single member, according to UniProt)

    • Structure

  • [PDB|1TYG] (complex with [protein|B58B8F260E446E28BC2E3A9AA84EA771EC0A8634|ThiG]) [Pubmed|15362849]

    • Expression and Regulation

    Operons

    genes
    [gene|47E47FB7D9C079DF603906694C4649A049814346|tenA]-[gene|CA6AAE8AC5B5EF01A911B035F8782579CD876C08|tenI]-[gene|87B5C84B7EE10F60B545CCFEF6A527A546B80432|thiO]-[gene|95DA3F899037140F48B99E065C768356CE08FA94|thiS]-[gene|B58B8F260E446E28BC2E3A9AA84EA771EC0A8634|thiG]-[gene|CCD3DDE9E52FCD4906735059CF30593BEB56FD1B|thiF]-[gene|97EDB4ACD64C5FA2E6014ED133DF18F602FF58BB|thiD]
    description
    [Pubmed|1898926]

    regulatory mechanism

  • [protein|E195900D971AEE4A790D4579BE5E5A15B81010B8|Thi-box]: [SW|RNA switch], via [SW|RNA switch], in [regulon|E195900D971AEE4A790D4579BE5E5A15B81010B8|Thi-box regulon]

    • regulation

  • repressed by thiamine ([SW|Thi-box]) [Pubmed|16356850]
  • the [SW|Thi-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]
    • view in new tab

    Biological materials

    Mutant

  • BKE11680 ([gene|95DA3F899037140F48B99E065C768356CE08FA94|thiS]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE11680 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GTCTTTACCGTTCAGCTGTA, downstream forward: _UP4_ATTGTCCATTTTGTAGGAGG
  • BKK11680 ([gene|95DA3F899037140F48B99E065C768356CE08FA94|thiS]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK11680 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GTCTTTACCGTTCAGCTGTA, downstream forward: _UP4_ATTGTCCATTTTGTAGGAGG

    • References

    Reviews

  • 19348578,10382260

    • Original publications

  • 15362849,14567704