sporulation-specific diadenylate cyclase, synthesis of c-di-AMP
function
synthesis of c-di-AMP
product
sporulation-specific diadenylate cyclase
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.3|Metabolism of signalling nucleotides][category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other][SW|Categories] containing this gene/protein
[SW|metabolism of signalling nucleotides], [SW|sporulation proteins]This gene is a member of the following [SW|regulons]
[SW|SigG regulon]Gene
Coordinates on the chromosome (coding sequence)
2,118,504 -> 2,119,127
The protein
Catalyzed reaction/ biological activity
synthesis of c-di-AMP from two molecules of ATP [Pubmed|23192352]Paralogous protein(s)
[protein|search|CdaA][SW|Domains]
N-terminal autoinhibitory domain [Pubmed|24939848]C-terminal [SW|DAC domain] involved in the synthesis of c-di-AMP [Pubmed|21566650]Structure
[PDB|2FB5] (the protein of ''B. cereus'', 48% identity, 81% similarity)[SW|Interactions]
forms hexamers (enzymatically inactive) or dimers (active) [Pubmed|24939848]Expression and Regulation
Operon
''[protein|search|cdaS]'' [Pubmed|22383849][SW|Sigma factor]
[protein|search|SigG] [Pubmed|22383849]Regulation
expressed late during sporulation in the forespore ([protein|search|SigG]) [Pubmed|22383849]Biological materials
Mutant
GP983 (''[protein|search|cdaS]''::''ermC''), available in [SW|Jörg Stülke]'s labGP1360 (''[protein|search|cdaS]''::''spc''), available in [SW|Jörg Stülke]'s labExpression vector
IPTG inducible expression of '''''B. cereus''''' Strep-''cdaS'' in ''E. coli'': pGP2593 (in [SW|pGP172]), available in [SW|Jörg Stülke]'s labReferences
Reviews
25869574,18714086,23812326 Original publications
21566650,23192352,22383849,24939848,26441857 
