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ispE
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ispE

4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, fourth step in the MEP pathway of isoprenoid biosynthesis
locus
BSU_00460
pI
6.35
mw
31.57 kDa
protein length
289 aa Sequence Blast
gene length
870 bp Sequence Blast
function
MEP pathway of isoprenoid biosynthesis
product
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
essential
yes
ec
2.7.1.148
synonyms
yabH
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.2|Biosynthesis of lipids] → [category|SW 2.4.2.3|Biosynthesis of isoprenoids]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

    • Gene

    Coordinates
    53,516 54,385

    Phenotypes of a mutant

  • essential [Pubmed|12682299]

    • The protein

    Catalyzed reaction/ biological activity

  • Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol (according to UniProt)
  • 4-CDP-2-C-methyl-D-erythritol + ATP --> 4-CDP-2-C-methyl-D-erythritol 2-phosphate + ADP + H+ (according to UniProt)

    • Protein family

  • GHMP kinase family (together with [protein|36930CC6FDFCA23D43E264FEA375B78A1434E1BD|GalK] and [protein|290302BA8A4E44C62AE3071C1F7DE18B20605607|ThrB]) (according to UniProt)

    • Structure

  • [PDB|1UEK] (from ''E. coli'', 38% identity) [Pubmed|12771135]

    • Expression and Regulation

    Operons

    genes
    [gene|8FA635D1915108BF29A864AF4DA28ECF633F735F|ispE]-[gene|A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|purR]-[gene|285D9E676B06119F9F4D33FC62615EC844D35BC3|yabJ]
    description
    [Pubmed|22383849]

    regulatory mechanism

  • [protein|A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|PurR]: repression, [Pubmed|7638212], in [regulon|A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|PurR regulon]

    • regulation

  • negative autoregulation [Pubmed|7638212]
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    Biological materials

    Mutant

  • MGNA-B910 (yabH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1909 NBRP B. subtilis, Japan]

    • References

  • 17458547,23840410,12771135,29335298