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ftsW [2020-05-28 15:25:49]
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ftsW [2020-05-28 15:25:49]

SEDS family peptidoglycan glycosyltransferase, cell-division protein
locus
BSU_14850
pI
9.72
mw
43.57 kDa
protein length
403 aa Sequence Blast
gene length
1212 bp Sequence Blast
function
cell division
product
SEDS family peptidoglycan glycosyltransferase
essential
yes
synonyms
ylaO
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.8|Cell division] → [category|SW 1.1.8.2|Other genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    1,552,899 1,554,110

    Phenotypes of a mutant

  • essential [Pubmed|12682299]

    • The protein

    Catalyzed reaction/ biological activity

  • principal peptidoglycan glycosyltransferase of the [SW|divisome] [Pubmed|27525505]
  • [GlcNAc-(1→4)-Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)](n)-diphospho-di-trans,octa-cis-undecaprenol + β-D-GlcNAc-(1→4)-Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)-diphospho-di-trans,octa-cis-undecaprenol --> [GlcNAc-(1→4)-Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)](n+1)-diphospho-di-trans-octa-cis-undecaprenol + di-trans,octa-cis-undecaprenyl diphosphate + H+ (according to UniProt)

    • Protein family

  • [SW|SEDS proteins] (shape, elongation, division, and sporulation) [Pubmed|27525505]
  • SEDS family (with [protein|B405B3C21B464F904BBB2AFD5DA21ADE45B4DD96|RodA] and [protein|5DE602D97D903D9AE38ED05D5A5F1B3A2DC1D58E|SpoVE], according to UniProt)

    • Paralogous protein(s)

  • [protein|B405B3C21B464F904BBB2AFD5DA21ADE45B4DD96|RodA], [protein|5DE602D97D903D9AE38ED05D5A5F1B3A2DC1D58E|SpoVE]

    • Structure

  • [PDB|6BAR] (from Thermus thermophilus,corresponds to aa 97... 359 34% identity) [pubmed|29590088]

    • [SW|Localization]

  • integral membrane protein
  • localizes to the division site [Pubmed|27895631]
  • localization depends on [protein|41872E2EF00C79918DD077F2EF78F37E24FEB110|FtsZ] [Pubmed|26679002]

    • Expression and Regulation

    Operons

    genes
    [gene|8AB7D225DBEE6BD695A4A8A2384D2C029B571C57|ftsW]-[gene|6ABF79C6EEF078E4F71F569FACCA80DA67191AE2|pycA]
    description
    [pubmed|22383849]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|20081037], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|6FDA046DCBBAE4588C4BC7163A04B9E6AEDBAC55|YofA]: activation, [Pubmed|17526699], in [regulon|6FDA046DCBBAE4588C4BC7163A04B9E6AEDBAC55|YofA regulon]
  • [regulon|stringent response|stringent response]: positive regulation, due to presence of adenines at +1 and +2 positions of the transcript [Pubmed|20081037], in [regulon|stringent response|stringent response]
  • [protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA]: repression, in [regulon|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA regulon]

    • regulation

  • constitutively expressed [Pubmed|23701187]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-A563 (ftsW::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/563 NBRP B. subtilis, Japan]

    • FLAG-tag construct

  • GP2007 ''ftsW-3xFLAG ermC'' (based on [SW|pGP1087]), available in [SW|Jörg Stülke]'s lab

    • References

    Reviews

  • 26679002,28975672

    • Original Publications

  • 17981970,19429628,17526699,21564336,23701187,27525505,27895631,17379809,27895631,29590088,30692671