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yfhK [2019-09-05 12:14:47]
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yfhK [2019-09-05 12:14:47]

general stress protein, similar to cell division inhibitor
locus
BSU_08570
pI
9.96
mw
18.55 kDa
protein length
172 aa Sequence Blast
gene length
519 bp Sequence Blast
function
survival of ethonol stress and at low and high temperatures
product
unknown
essential
no
synonyms

Genomic Context

      

categories

  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]
  • Gene

    Coordinates
    928,803 929,321

    The protein

    [SW|Domains]

  • [SW|SH3B domain] 1 (aa 42-105) (according to UniProt)
  • [SW|SH3B domain] 2 (aa 112-172) (according to UniProt)
  • Expression and Regulation

    Operons

    genes
    [gene|893103B8E6163A4B403CA136A92BA3EA66DC3802|yfhK]-[gene|2B7F65B145005A8ADD2A278E3EA5A52DF162C837|yfhL]-[gene|ED478E2C37F2AD8BF06251B52511287EDB91C9EE|yfhM]
    description
    [Pubmed|10913081]

    sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|10913081,15805528], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]
  • [protein|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW]: sigma factor, [Pubmed|9987136], in [regulon|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW regulon]
  • regulation

  • induced by stress ([protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]) [Pubmed|10913081,15805528]
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    Biological materials

    Mutant

  • MGNA-C317 (yfhK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2315 NBRP B. subtilis, Japan]
  • BKE08570 ([gene|893103B8E6163A4B403CA136A92BA3EA66DC3802|yfhK]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE08570 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATAAAGCTCCTCCTCGC, downstream forward: _UP4_TAAAAAGGCAGGCCGAGGCT
  • BKK08570 ([gene|893103B8E6163A4B403CA136A92BA3EA66DC3802|yfhK]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK08570 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATAAAGCTCCTCCTCGC, downstream forward: _UP4_TAAAAAGGCAGGCCGAGGCT
  • References

  • 12107147,15805528,10913081,9987136