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pbpH [2020-04-05 12:52:50]
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pbpH [2020-04-05 12:52:50]

class B penicillin-binding protein H, required for [SW|cell wall synthesis ]during cell elongation
locus
BSU_13980
pI
8.89
mw
76.98 kDa
protein length
685 aa Sequence Blast
gene length
2058 bp Sequence Blast
function
formation of a rod-shaped peptidoglycan cell wall
product
penicillin-binding protein H
essential
no
synonyms
ykuA
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.7|Penicillin-binding proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    1,467,805 1,469,862

    The protein

    Catalyzed reaction/ biological activity

  • required for [SW|cell wall synthesis] during cell elongation [Pubmed|12896990]
  • driver of [protein|A4C8719E06F774A6EB4D79757CC79CF89E453A54|MreB] dynamics [Pubmed|21636744,21636745]

    • Protein family

  • [SW|transpeptidase family] (according to UniProt)

    • Paralogous protein(s)

  • [protein|3B4F035535D6504405567E7C44E72902A11F7447|PbpA]

    • Structure

  • [PDB|2WAF] (Pbp2B from Streptococcus pneumoniae, 31% identity) [pubmed|19233207]

    • [SW|Localization]

  • during vegetative growth: evenly distributed along the membrane [pubmed|14731276]
  • localization depends on the availability of peptidoglyan precursors (lipid II) [Pubmed|23895585]

    • Expression and Regulation

    Operons

    genes
    [gene|883DFF72888D280A102E53CC1D69A8B1C7BE2907|pbpH]
    description
    [pubmed|22383849]

    regulatory mechanism

  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|20817675], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]
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    Biological materials

    Mutant

  • MGNA-B335 (ykuA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1334 NBRP B. subtilis, Japan]
  • BKE13980 ([gene|883DFF72888D280A102E53CC1D69A8B1C7BE2907|pbpH]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE13980 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTGATTGCTCTGTTTC, downstream forward: _UP4_TAAAAAACAGGGTGCACAAC
  • BKK13980 ([gene|883DFF72888D280A102E53CC1D69A8B1C7BE2907|pbpH]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK13980 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTGATTGCTCTGTTTC, downstream forward: _UP4_TAAAAAACAGGGTGCACAAC

    • GFP fusion

  • 3140 [gene|0467447B10EE7C1FDF86022AB6FBDBABFD32A9E5|trpC]2 [gene|883DFF72888D280A102E53CC1D69A8B1C7BE2907|pbpH]::pSG5058 (cat Pxyl-gfpa-[gene|883DFF72888D280A102E53CC1D69A8B1C7BE2907|pbpH]1-827) [pubmed|14731276], available in [SW|Dirk Jan Scheffers]' lab and in the [http://bgsc.org BGSC]

    • References

  • 12896990,20525796,21636744,21636745,20817675,23199363,23895585,14731276,19233207