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ytpA [2019-06-19 10:50:39]
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ytpA [2019-06-19 10:50:39]

phospholipase (annotated as a bacilysocin synthase)
locus
BSU30510
pI
6.77
mw
29.42 kDa
protein length
259 aa Sequence Blast
gene length
780 bp Sequence Blast
function
lipid degradation
product
phospholipase
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]
  • [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

    • Gene

    Coordinates
    3,122,662 3,123,441

    The protein

    Catalyzed reaction/ biological activity

  • cleaves fatty acids from the 2 position of phosphatidylglycerol [Pubmed|11796336]

    • Structure

  • [PDB|3HJU] (human monoglyceride lipase, 30% identity) [Pubmed|19957260]

    • Expression and Regulation

    Operons

    genes
    [gene|85A653AF4752A61A39F9C42FC791B2D1444886B1|ytpA]-[gene|596C09DD1D9C305B7C49B2F5E90E2C6C33F63D0E|ytpB]
    description
    [Pubmed|18179421]

    sigma factors

  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]: sigma factor, [Pubmed|18179421], in [regulon|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM regulon]

    • regulation

  • expressed under conditions of salt stress ([protein|search|SigM]) [http://www.ncbi.nlm.nih.gov/sites/entrez/18179421 Pubmed]
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    Biological materials

    Mutant

  • MGNA-A128 (ytpA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/128 NBRP B. subtilis, Japan]
  • BKE30510 ([gene|85A653AF4752A61A39F9C42FC791B2D1444886B1|ytpA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE30510 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATCTTCACCTCATACG, downstream forward: _UP4_TGAACTAGTAGGGGGTGCAT
  • BKK30510 ([gene|85A653AF4752A61A39F9C42FC791B2D1444886B1|ytpA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK30510 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATCTTCACCTCATACG, downstream forward: _UP4_TGAACTAGTAGGGGGTGCAT

    • References

  • 18179421,11796336,19957260