DNA polymerase III (beta subunit), beta clamp, part of the replisome, also involved in DNA mismatch repair, inhibitor of DnaA oligomerization
function
DNA replication, DNA repair
product
DNA polymerase III
(beta subunit), beta clamp
Genomic Context
categories
[category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.1|DNA replication][category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.7|Mismatch repair (MMR)][category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes][SW|Categories] containing this gene/protein
[SW|DNA replication], [SW|essential genes]This gene is a member of the following [SW|regulons]
[SW|Spo0A regulon]Gene
Coordinates on the chromosome (coding sequence)
1,939 -> 3,075
Phenotypes of a mutant
essential [Pubmed|12682299]The protein
Catalyzed reaction/ biological activity
[protein|search|DnaN] overexpression or release from the [SW|replisome] decreases association of [protein|search|YabA] with ''oriC'', increases association of [protein|search|DnaA] with ''oriC'' [Pubmed|21895792]Deoxynucleoside triphosphate DNA(n) = diphosphate DNA(n 1) (according to Swiss-Prot)recruits [protein|search|MutS] to the site of [SW|DNA replication] ([SW|replisome]) [Pubmed|23228104]required for bacteriophage SPP1 replication [Pubmed|23268446]inhibits oligomerization and helix formation of [protein|search|DnaA] [Pubmed|23909787]Structure
[PDB|2AWA] (from ''Streptococcus pneumoniae'', 41% identity, 77% similarity)[SW|Localization]
nucleoid (mid-cell spot) [Pubmed|9822387] [Pubmed|16479537]forms large assemblies on DNA, called clamp zones [Pubmed|21419346]forms foci [Pubmed|21958350][SW|Interactions]
[protein|search|DnaA]-[protein|search|YabA]-[protein|search|DnaN] [Pubmed|12060778]part of the [SW|replisome]: [protein|search|PolC]-[protein|search|HolA]-[protein|search|HolB]-[protein|search|DnaX]-[protein|search|DnaN]-[protein|search|DnaG]-[protein|search|DnaC]-[protein|search|DnaI]-[protein|search|DnaD]-[protein|search|SsbA]-[protein|search|DnaE]-[protein|search|PriA]-[protein|search|DnaB] [Pubmed|20122408][SW|PolY1]-[protein|search|DnaN], [SW|PolY2]-[protein|search|DnaN] (required for untargeted mutagenesis) [Pubmed|15469515][SW|PolY1]-[protein|search|PolA]-[protein|search|DnaN] [Pubmed|16045613][protein|search|MutL] (C-terminal domain)-[protein|search|DnaN], the interaction stimulates the endonuclease activity of [SW|MutL] [Pubmed|26384423,21050827][protein|search|MutS]-[protein|search|DnaN] [Pubmed|20453097][protein|search|DnaE]-[protein|search|DnaN] [Pubmed|21958350][protein|search|DnaN] forms dimers [Pubmed|16045613]Expression and Regulation
Operon
''[protein|search|dnaA]-[protein|search|dnaN]'' [Pubmed|2987848][SW|Sigma factor]
[protein|search|SigA] [Pubmed|2987848]Regulation
negatively controlled by [protein|search|DnaA] [Pubmed|2168872] and [SW|Spo0A] [Pubmed|14651647]repressed under conditions that trigger sporulation ([SW|Spo0A]) [Pubmed|14651647]Regulatory mechanism
[SW|Spo0A]: transcription repression [Pubmed|14651647]Biological materials
GFP fusion
[Pubmed|16942601] [Pubmed|9822387]two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Fabian Commichau]'s labLabs working on this gene/protein
[SW|Philippe Noirot], Jouy-en-Josas, France [http://locus.jouy.inra.fr/cms/index.php?id=18 homepage]References
Reviews
20157337,22933559,26343983 Original publications
16942601,9822387,14651647,2987848,2168872,14651647,12060778,16461910,16479537,19737352,19081080,11395445,20122408,20451384,15469515,16045613,21895792,20453097,23228104,23268446,21050827,21419346,21958350,23909787,25170813,26384423,26615189,27369079 
