fructose 1,6-bisphosphate aldolase, glycolytic/ gluconeogenic enzyme
function
enzyme in glycolysis/ gluconeogenesis
product
fructose-1,6-bisphosphate aldolase
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.1|Glycolysis][category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.2|Gluconeogenesis][category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.6|Phosphorylation on a Thr residue]Gene
Coordinates
3,808,512 3,809,369
Phenotypes of a mutant
essential [Pubmed|12682299], non-essential according to [Pubmed|23420519]The protein
Catalyzed reaction/ biological activity
β-D-fructose 1,6-bisphosphate --> D-glyceraldehyde 3-phosphate + dihydroxyacetone phosphate (according to UniProt)Protein family
class II fructose-bisphosphate aldolase family (with [protein|1EFB5C8F28146D1EA3B615F548D0CF262D2B4DF5|IolJ], according to UniProt)Paralogous protein(s)
[protein|1EFB5C8F28146D1EA3B615F548D0CF262D2B4DF5|IolJ] [SW|Domains]
2 x Dihydroxyacetone phosphate binding domain (210212), (231234)Modification
phosphorylation on Thr-212 and Thr-234 [Pubmed|17218307][SW|Cofactors]
Zn2+ (Metalloenzyme)Effectors of protein activity
Inhibited by alpha-ketoglutarate, oxaloacetate and pyruvate [Pubmed|24624] [Pubmed|15125960]Activated by NH4+ [Pubmed|15125960]Structure
[PDB|3Q94] (from ''Bacillus anthracis'')Additional information
Binds 2 zinc ions per subunit. One is catalytic and the other provides a structural contributionextensive information on the structure and enzymatic properties of FbaA can be found at [http://www.proteopedia.org/wiki/index.php/Fructose_Bisphosphate_Aldolase Proteopedia]belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]Expression and Regulation
Operons
genes
[gene|800EC1F1CA8C4F2A643EDECBB684347C29CAB0CA|fbaA]
description
[Pubmed|11489127]
regulation
constitutively expressed [Pubmed|11489127]view in new tabgenes
[gene|800EC1F1CA8C4F2A643EDECBB684347C29CAB0CA|fbaA]-[gene|1419F2F99D924973D75EE6FAA41F9A27EC5283E1|ywjH]
description
[Pubmed|11489127]
regulation
constitutively expressed [Pubmed|11489127]view in new tabBiological materials
Mutant
GP591 (''fbaA''::''cat''), available in [SW|Jörg Stülke]'s lab, [Pubmed|23420519]GP596 (''fbaA''::''erm''), available in [SW|Jörg Stülke]'s lab, [Pubmed|23420519]BKE37120 ([gene|800EC1F1CA8C4F2A643EDECBB684347C29CAB0CA|fbaA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE37120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCGAATGTCCTCCTTA, downstream forward: _UP4_TAATTCAATTGGAACTTTTTBKK37120 ([gene|800EC1F1CA8C4F2A643EDECBB684347C29CAB0CA|fbaA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK37120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCGAATGTCCTCCTTA, downstream forward: _UP4_TAATTCAATTGGAACTTTTTExpression vectors
for expression in ''B. subtilis'', in [SW|pBQ200]: pGP1423, available in [SW|Jörg Stülke]'s labfor expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [SW|SPINE], in [SW|pGP380]: pGP88, available in [SW|Jörg Stülke]'s lab, [pubmed|19193632]for expression/ purification from ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP395, available in [SW|Jörg Stülke]'s lablacZ fusion
pGP601 (in [SW|pAC6]), available in [SW|Jörg Stülke]'s lab [pubmed|11489127]two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab [pubmed|19193632]References
Reviews
Original publications
17218307,15125960,24624,16843441,11489127,20525796,23420519,23033921,24571712,15378759