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topB

DNA topoisomerase III, decatenase
locus
BSU_04260
pI
9.88
mw
81.29 kDa
protein length
727 aa Sequence Blast
gene length
2184 bp Sequence Blast
function
control of DNA recombination, maintenance of genomic stability
product
DNA topoisomerase III
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.1|DNA replication]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.3|DNA condensation/ segregation]

    • Gene

    Coordinates
    476,558 478,741

    Phenotypes of a mutant

  • in E. coli, deletion results in a mutator phenotype [pubmed|2016043,1321123]

    • The protein

    Catalyzed reaction/ biological activity

  • ATP-independent breakage of single-stranded DNA, followed by passage and rejoining (according to UniProt)

    • Protein family

  • Type IA topoisomerase family (together with [protein|7D5327BD1DD5FD5F90B2C849589923AA3D8B20EF|TopB])(according to UniProt)

    • Paralogous protein(s)

  • [protein|41199C76DF8CA804B7B8878D61228B9542A96AE4|TopA]

    • [SW|Domains]

  • N-terminal [SW|TOPRIM domain] (aa 3-136) [Pubmed|9722641]

    • Effectors of protein activity

  • [protein|search|RecQ ]controls [protein|7D5327BD1DD5FD5F90B2C849589923AA3D8B20EF|Topo III] (de)catenation activity [pubmed|11497238,10360177]

    • Structure

  • [PDB|1D6M] (from ''E. coli'', 33% identity) [Pubmed|10574789]

    • Expression and Regulation

    Operons

    genes
    [gene|7D5327BD1DD5FD5F90B2C849589923AA3D8B20EF|topB]

    description

  • [pubmed|22383849]
    • view in new tab

    genes
    [gene|AC2618C53DA020F2CD5192AEE462D15E8EE0F57F|lrpC]-[gene|7D5327BD1DD5FD5F90B2C849589923AA3D8B20EF|topB]

    description

  • [pubmed|22383849]

    • sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [pubmed|10913073], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-C111 (topB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2109 NBRP B. subtilis, Japan]
  • GP1864 (Δ''topB''::''ermC'') (available in [SW|Jörg Stülke]'s lab)
  • GP1896 (Δ''topB''::''cat'') (available in [SW|Jörg Stülke]'s lab)
  • JH642 and its derivatives: natural deletion of the 18 kb [gene|467E9CF20B545A976614968E7DD5497F9390D344|ydzA]-[gene|353DADE8E57A0F1895CCEB62701F9273EEB5EB45|mntH] region encompassing the genes [gene|467E9CF20B545A976614968E7DD5497F9390D344|ydzA]-[gene|7D5327BD1DD5FD5F90B2C849589923AA3D8B20EF|topB]-[gene|69DEE29383E40F49890CC8314DFBF56D70D35113|ydaJ]-[gene|70ED3CE683F3A3F785480F40986CABE7729C17C7|ydaK]-[gene|28D80C5497AE29A24AD6B9A1A713A2B3915F2CED|ydaL]-[gene|961538BEDD27C24FC073AFB7AB3268E3D46783CC|ydaM]-[gene|ADE0122D8FFF2F78B6D21031B7D02A323A13ED63|ydaN]-[gene|5968E6D4D7378C69EC3E1E05B285069123AA1FD3|kimA]-[gene|6085BDAD40F719555EA4EEA7BBAD74103BD03D0F|mutT]-[gene|2883FC60CD69948D5C43BDDFEFBE7A1CA7C743C7|ydaP]-[gene|87834D5C47064BEFF26F086A8202C311F28F9D38|ydzK]-[gene|search|mntH ][pubmed|18670626]
  • BKE04260 (Δ[gene|7D5327BD1DD5FD5F90B2C849589923AA3D8B20EF|topB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE04260 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCTTGATCACTTCTCTT, downstream forward: _UP4_TAATAAAAAAACCTGTGCCT
  • BKK04260 (Δ[gene|7D5327BD1DD5FD5F90B2C849589923AA3D8B20EF|topB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK04260 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCTTGATCACTTCTCTT, downstream forward: _UP4_TAATAAAAAAACCTGTGCCT

    • References

    Reviews

  • 11497238,6305585,16936698,29177730

    • Original publications

  • 17114254,16600296,9722641,10574789,10360177,25232096,2016043,1321123,25232096,23514156,22923519,12167668,30222737,30478267,30957856