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ymcB [2019-08-01 11:22:31]
tRNA methylthiotransferase
product
tRNA methylthiotransferase
Genomic Context
categories
[category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.8|tRNA modification and maturation]Gene
Coordinates
1,772,843 1,774,372
The protein
Catalyzed reaction/ biological activity
modifies N(6)-isopentenyladenosine (i(6)A) to 2-methylthio-N(6)-isopentenyladenosine (ms(2)i(6)A) in tRNA [Pubmed|20472640]Protein family
methylthiotransferase family (with [protein|6ADA5AF308D96218414EA92DFF4A57BAEAD0554B|YqeV], according to UniProt)Paralogous protein(s)
[protein|6ADA5AF308D96218414EA92DFF4A57BAEAD0554B|YqeV] [SW|Cofactors]
Fe-S cluster [pubmed|29292548]Structure
[PDB|4JC0] (from Thermotoga maritima, 33% for aa 70 ... 504) [pubmed|23542644][SW|Localization]
cytoplasm (according to Swiss-Prot)Expression and Regulation
Operons
genes
[gene|7C9F99FF1BCDB0E1CFB0AFE959C7F16A99562E18|ymcB]-[gene|EE52DFA35B935E551871D079A9BE877DB2001A3B|ymcA]
description
[Pubmed|22383849]
Biological materials
Mutant
GP1109 (spc), available in [SW|Jörg Stülke]'s labBKE17010 ([gene|7C9F99FF1BCDB0E1CFB0AFE959C7F16A99562E18|ymcB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE17010 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCATATTTTCTCCTTT, downstream forward: _UP4_GGAGAAGCAATCGAGGTGAABKK17010 ([gene|7C9F99FF1BCDB0E1CFB0AFE959C7F16A99562E18|ymcB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK17010 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCATATTTTCTCCTTT, downstream forward: _UP4_GGAGAAGCAATCGAGGTGAAReferences
