UDP-glucose diacylglycerol glucosyltransferase, growth-rate dependent inhibitor of cell division
function
synthesis of glucolipids and anchoring of lipoteichoic
acid, inhibition of FtsZ assembly
product
UDP-glucose diacylglycerol glucosyltransferase
Genomic Context
categories
[category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.2|Cell shape][category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.8|Cell division] → [category|SW 1.1.8.2|Other genes][category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.3|Lipid metabolism/ other][category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)][category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins][SW|Categories] containing this gene/protein
[SW|cell division], [SW|lipid metabolism/ other], [SW|cell envelope stress proteins (controlled by SigM, V, W, X, Y)], [SW|membrane proteins]This gene is a member of the following [SW|regulons]
[SW|SigM regulon]Gene
Coordinates on the chromosome (coding sequence)
2,306,514 -> 2,307,662
Phenotypes of a mutant
cells are bent and distended due to the lack of glucolipids [Pubmed|22362028,27684739]increased expression of the [protein|search|SigM], [protein|search|SigV], and [protein|search|SigX] regulons [Pubmed|22362028]altered [SW|localization] of [protein|search|MreB] (irregular clusters instead of helical dots) [Pubmed|22362028]the inactivation of ''[protein|search|ugtP]'' suppresses the poor and filamentous growth of the ''[protein|search|yvcL] [protein|search|zapA]'' double mutant [Pubmed|24097947]increased conjugation of ICEBs1 [Pubmed|25069588]The protein
Catalyzed reaction/ biological activity
UDP-glucose 1,2-diacylglycerol = UDP 1,2-diacyl-3-(O-beta-D-glucopyranosyl)-sn-glycerol (according to Swiss-Prot)the interaction with [protein|search|FtsZ] results in inhibition of [SW|cell division] and an increase of cell size [Pubmed|22931116] Effectors of protein activity
oligomerization of [protein|search|UgtP] is inhibited by UDP-Glc and by interaction with [protein|search|FtsZ] [Pubmed|22931116][SW|Localization]
membrane-bound protein, self-assembles into tightly wound spirals ''in vitro'' [Pubmed|17662947]under nutrient rich conditions (increased concentration of UDP-Glc): throughout the cell, concentrated at the cell poles and/or the cytokinetic ring, interaction with [protein|search|FtsZ] [Pubmed|22931116]under nutrient poor conditions: forms punctate foci (oligomers), no interaction with [protein|search|FtsZ] [Pubmed|22931116][SW|Interactions]
[protein|search|EzrA]-[protein|search|UgtP] [Pubmed|17662947][protein|search|UgtP]-[protein|search|UgtP] [Pubmed|17662947], forms oligomers [Pubmed|22931116][protein|search|UgtP]-[protein|search|FtsZ] (under nutrient rich conditions) [Pubmed|22931116]Expression and Regulation
Operon
''[protein|search|metA]-[protein|search|ugtP]'' [Pubmed|22383849]''[protein|search|ugtP]'' [Pubmed|22383849][SW|Sigma factor]
''[protein|search|ugtP]'': [protein|search|SigM] [Pubmed|18179421]Biological materials
Mutant
MGNA-A886 (ypfP::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/886 NBRP B. subtilis, Japan]GP1369 (''[protein|search|ugtP]''::''spc''), available in [SW|Jörg Stülke]'s labExpression vector
pGP2571, for expression in ''B. subtilis'' (based on [SW|pBQ200], available in [SW|Jörg Stülke]'s labpGP2600, for expression/ purification from ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172], available in [SW|Jörg Stülke]'s labReferences
Reviews
19680248,17662935,22575476 Original Publications
9244290,18820022,17662947,9720862,22362028,15640167,23935518,22931116,24097947,25069588,27684739 
