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pckA [Mon Aug 31 2015 13:56:24 GMT+0200 (CEST)]
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pckA [Mon Aug 31 2015 13:56:24 GMT+0200 (CEST)]

phosphoenolpyruvate carboxykinase
locus
BSU30560
pI
5.12
mw
58.10 kDa
function
synthesis of phosphoenolpyruvate
product
phosphoenolpyruvate carboxykinase
essential
no
ec
4.1.1.49
synonyms
ppc
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.2|Gluconeogenesis]
    • syns
    ppc

    [SW|Categories] containing this gene/protein

  • [SW|carbon core metabolism]

    • This gene is a member of the following [SW|regulons]

  • [SW|CcpN regulon]

    • The protein

    Catalyzed reaction/ biological activity

  • ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO2 (according to Swiss-Prot) ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO(2)

    • Protein family

  • phosphoenolpyruvate carboxykinase [ATP] family (according to Swiss-Prot) phosphoenolpyruvate carboxykinase [ATP] family

    • [SW|Domains]

  • Nucleotide binding Domain (233–240)

    • Structure

  • [PDB|2PXZ] (''E.coli'')

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot), cytoplasm

    • [SW|Interactions]

  • [protein|search|Mdh]-[protein|search|PckA] (this interaction occurs under gluconeogenic, but not under glycolytic conditions) [Pubmed|20933603]

    • Expression and Regulation

    Operon

  • ''[protein|search|pckA]'' [Pubmed|9387221]

    • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|15720552]

    • Regulation

  • repressed by glucose (35-fold) [protein|search|CcpN] [Pubmed|15720552,12850135]

    • Regulatory mechanism

  • [protein|search|CcpN]: transcription repression [Pubmed|15720552]

    • Biological materials

    Mutant

  • 1A1005 ( ''pckA''::''spec''), [Pubmed| ], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A1005&Search=1A1005 BGSC]
  • 1A996 ( ''pckA''::''spec''), [Pubmed| ], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A996&Search=1A996 BGSC]
  • GP1147 (''pckA''::''neo''), available in [SW|Jörg Stülke]'s lab

    • Expression vector

  • pGP1753 (N-terminal Strep-tag, for [SW|SPINE], purification from ''B. subtilis'', in [SW|pGP380]) (available in [SW|Jörg Stülke]'s lab)
  • pGP1762 (for expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844], available in [SW|Jörg Stülke]'s lab)
  • pGP1763 (for expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172], available in [SW|Jörg Stülke]'s lab)

    • GFP fusion

  • GP1430 (spc, based on [SW|pGP1870]), available in the [SW|Stülke] lab

    • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

    • FLAG-tag construct

  • GP1129 (spc, based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab

    • Antibody

  • **

    • Labs working on this gene/protein

  • [SW|Stephane Aymerich], Microbiology and Molecular Genetics, INRA Paris-Grignon, France

    • References

  • 12850135,15720552,18586936,9387221,20933603,22190493,23136871