SubtiBank SubtiBank
ydaJ
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ydaJ

lipoprotein, may modify the extracellular polysaccharide synthesized by [protein|28D80C5497AE29A24AD6B9A1A713A2B3915F2CED|YdaL]-[protein|961538BEDD27C24FC073AFB7AB3268E3D46783CC|YdaM]-[protein|ADE0122D8FFF2F78B6D21031B7D02A323A13ED63|YdaN]
locus
BSU_04270
pI
4.90
mw
40.84 kDa
protein length
362 aa Sequence Blast
gene length
1089 bp Sequence Blast
function
unknown
product
lipoprotein
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]
  • [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]

    • Gene

    Coordinates
    478,944 480,032

    The protein

    Protein family

  • glycosyl hydrolase 8 (GH8) family [Pubmed|27897378]

    • Structure

  • [PDB|3REN] (alpha-amylase from Clostridium perfringens, 25% identity) [pubmed|21905105]

    • [SW|Localization]

  • exported with N-terminal signal peptide, attached to the cell membrane (lipoprotein), faces towards the medium [Pubmed|27897378]

    • Expression and Regulation

    Operons

    genes
    [gene|69DEE29383E40F49890CC8314DFBF56D70D35113|ydaJ]-[gene|70ED3CE683F3A3F785480F40986CABE7729C17C7|ydaK]-[gene|28D80C5497AE29A24AD6B9A1A713A2B3915F2CED|ydaL]-[gene|961538BEDD27C24FC073AFB7AB3268E3D46783CC|ydaM]-[gene|ADE0122D8FFF2F78B6D21031B7D02A323A13ED63|ydaN]
    description
    [Pubmed|22383849]

    sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|22383849], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]

    • regulation

  • expressed during stress ([protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]) [Pubmed|22383849]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-C073 (ydaJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2071 NBRP B. subtilis, Japan]
  • JH642 and its derivatives: natural deletion of the 18 kb [gene|467E9CF20B545A976614968E7DD5497F9390D344|ydzA]-[gene|353DADE8E57A0F1895CCEB62701F9273EEB5EB45|mntH] region encompassing the genes [gene|467E9CF20B545A976614968E7DD5497F9390D344|ydzA]-[gene|7D5327BD1DD5FD5F90B2C849589923AA3D8B20EF|topB]-[gene|69DEE29383E40F49890CC8314DFBF56D70D35113|ydaJ]-[gene|70ED3CE683F3A3F785480F40986CABE7729C17C7|ydaK]-[gene|28D80C5497AE29A24AD6B9A1A713A2B3915F2CED|ydaL]-[gene|961538BEDD27C24FC073AFB7AB3268E3D46783CC|ydaM]-[gene|ADE0122D8FFF2F78B6D21031B7D02A323A13ED63|ydaN]-[gene|5968E6D4D7378C69EC3E1E05B285069123AA1FD3|kimA]-[gene|6085BDAD40F719555EA4EEA7BBAD74103BD03D0F|mutT]-[gene|2883FC60CD69948D5C43BDDFEFBE7A1CA7C743C7|ydaP]-[gene|87834D5C47064BEFF26F086A8202C311F28F9D38|ydzK]-[gene|search|mntH ][pubmed|18670626]
  • BKE04270 ([gene|69DEE29383E40F49890CC8314DFBF56D70D35113|ydaJ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE04270 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAGGCAAATCCCCCTTTG, downstream forward: _UP4_CTGTTAGCCGAAAGGAAGCT
  • BKK04270 ([gene|69DEE29383E40F49890CC8314DFBF56D70D35113|ydaJ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK04270 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAGGCAAATCCCCCTTTG, downstream forward: _UP4_CTGTTAGCCGAAAGGAAGCT

    • References

    Reviews

  • 28296273

    • Original publications

  • 22383849,27897378,21905105