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citA [2019-02-04 16:11:17]
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citA [2019-02-04 16:11:17]

minor citrate synthase
locus
BSU09440
pI
5.78
mw
40.78 kDa
protein length
366 aa Sequence Blast
gene length
1101 bp Sequence Blast
function
unknown
product
minor citrate synthase
essential
no
ec
2.3.3.1
synonyms
citA1
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

    • Gene

    Coordinates
    1,021,057 1,022,157

    The protein

    Catalyzed reaction/ biological activity

  • Acetyl-CoA + H2O + oxaloacetate = citrate + CoA (according to Swiss-Prot)

    • Protein family

  • citrate synthase family (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|3DB116F7423C31838ED6EE090B94DA5F93A4F40C|MmgD], [protein|58E3A065A95D03B4FB1F544906F53D1704D29EAD|CitZ]

    • Structure

  • [PDB|2C6X]

    • Expression and Regulation

    Operons

    genes
    [gene|6684F6083B001E9FDC5967799B715AE966B83E1D|citA]-[gene|EAEEA4DD9641919830A81185333A2610B964D37C|yhdF]
    description
    [Pubmed|22383849]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8045898], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|E7E0B170D1EEECE4E4EDC29B0399CB48253E9A17|CitR]: repression, [Pubmed|8045899], in [regulon|E7E0B170D1EEECE4E4EDC29B0399CB48253E9A17|CitR regulon]

    • regulation

  • induced by stress ([protein|search|SigB]) [Pubmed|11544224]
    • view in new tab

    Biological materials

    Mutant

  • GP676 (erm), available in [SW|Jrg Stlke]'s lab
  • GP1282 (''[gene|6684F6083B001E9FDC5967799B715AE966B83E1D|citA]''::''cat''), available in [SW|Jrg Stlke]'s lab
  • GP1753 (''[gene|E7E0B170D1EEECE4E4EDC29B0399CB48253E9A17|citR] [gene|6684F6083B001E9FDC5967799B715AE966B83E1D|citA]''::''aphA3''), the resistance cassette can be cut out by introducing the ''cre''-rekombinase into the chromosom of ''B. subtilis'', available in [SW|Jrg Stlke]'s lab
  • GP2360 (''[gene|E7E0B170D1EEECE4E4EDC29B0399CB48253E9A17|citR] [gene|6684F6083B001E9FDC5967799B715AE966B83E1D|citA]''::''erm''), the resistance cassette can be cut out by introducing the ''cre''-rekombinase into the chromosom of ''B. subtilis'', available in [SW|Jrg Stlke]'s lab
  • BKE09440 ([gene|6684F6083B001E9FDC5967799B715AE966B83E1D|citA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09440 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGTATTCCCCCTATCC, downstream forward: _UP4_TAACATATTTTGGCGTTTAT
  • BKK09440 ([gene|6684F6083B001E9FDC5967799B715AE966B83E1D|citA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09440 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGTATTCCCCCTATCC, downstream forward: _UP4_TAACATATTTTGGCGTTTAT

    • Expression vectors

  • pGP2516 (N-terminal Strep-tag, purification from ''E. coli'', in [SW|pGP172]), available in [SW|Jrg Stlke]'s lab

    • FLAG-tag construct

  • GP1287 (''spc'', based on [SW|pGP1331]), available in [SW|Jrg Stlke]'s lab

    • References

  • 8045899,8045898,22383849