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yeeF

polymorphic toxin, non-specific metal-dependent DNase

locus

BSU_06812

8.94

74.00 kDa

protein length

669 aa Sequence Blast

gene length

2010 bp Sequence Blast

function

competetion with other bacteria

product

non-specific metal-dependent DNase

essential

synonyms

Outlinks

KEGG BsubCyc SubtiList UniProt Expression Browser

Genomic Context

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categories

[category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.17|Toxins, antitoxins and immunity against toxins] → [category|SW 4.3.17.2|Type 2 TA systems]

[category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.18|Toxins, antitoxins and immunity against toxins/ based on similarity]

Gene

Coordinates

747,554 749,563

The protein

Catalyzed reaction/ biological activity

non-specific metal-dependent DNase activity [pubmed|32117125]

[SW|Domains]

[SW|LXG domain] (aa 1-235) (according to UniProt)

Expression and Regulation

Operons

genes

[gene|655C07294D3A04763049D6701A7296BB4AF8E6B9|yeeF]-[gene|9202D58FAC1B7B9CF693D537A12C683A3573B17E|yezG]

description

[pubmed|22383849]

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Biological materials

Mutant

MGNA-A963 (yeeF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/963 NBRP B. subtilis, Japan]

BKE06812 ([gene|655C07294D3A04763049D6701A7296BB4AF8E6B9|yeeF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE06812 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGACCATTTCCCTTCTTT, downstream forward: _UP4_TAGAAGAATGGAAACTAGGA

BKK06812 ([gene|655C07294D3A04763049D6701A7296BB4AF8E6B9|yeeF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK06812 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGACCATTTCCCTTCTTT, downstream forward: _UP4_TAGAAGAATGGAAACTAGGA

References

22200572,32117125