SubtiBank SubtiBank
pbuO [2018-05-04 10:22:29]
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pbuO [2018-05-04 10:22:29]

similar to hypoxanthine/guanine permease PbuG
locus
BSU29990
pI
7.48
mw
45.26 kDa
protein length
432 aa Sequence Blast
gene length
1296 bp Sequence Blast
function
unknown
product
unknown
essential
no
synonyms
ytiP
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.6|Nucleotide/ nucleoside transporter]
  • [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.2|Biosynthesis/ acquisition of nucleotides] → [category|SW 2.5.2.2|Biosynthesis/ acquisition of purine nucleotides/ based on similarity]

    • Gene

    Coordinates
    3,068,908 → 3,070,206

    The protein

    Paralogous protein(s)

  • [protein|41DDD3AAFE89D0F52AB5ABD08CEE30A1767FFD7A|PbuG]

    • Expression and Regulation

    Operons

    genes
    [gene|62AC66725C55B9E5FBBEB4EC43A88F7081B74D5C|pbuO]
    description
    [Pubmed|9387221]

    regulatory mechanism

  • [protein|A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|PurR]: repression, (molecular inducer: PRPP) [Pubmed|11591660,2536750], in [regulon|A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|PurR regulon]

    • regulation

  • repressed in the presence of purine nucleotides ([protein|search|PurR]) [Pubmed|11591660,2536750]
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    Biological materials

    Mutant

  • MGNA-A819 (ytiP::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/819 NBRP B. subtilis, Japan]
  • BKE29990 (Δ[gene|62AC66725C55B9E5FBBEB4EC43A88F7081B74D5C|pbuO]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29990 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAGTATCCTCCAATTACG, downstream forward: _UP4_TAAGGATAGACCAAAAAACC
  • BKK29990 (Δ[gene|62AC66725C55B9E5FBBEB4EC43A88F7081B74D5C|pbuO]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29990 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAGTATCCTCCAATTACG, downstream forward: _UP4_TAAGGATAGACCAAAAAACC

    • References

  • 11591660,2536750,9387221