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sppI
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sppI

antagonist of [protein|ACB7F015027A83C5D3457818A71B6F47192CEFBE|SppA], stress protein
locus
BSU_29520
pI
9.00
mw
18.98 kDa
protein length
164 aa Sequence Blast
gene length
495 bp Sequence Blast
function
inhibition of [protein|ACB7F015027A83C5D3457818A71B6F47192CEFBE|SppA] protease activtiy
product
[protein|ACB7F015027A83C5D3457818A71B6F47192CEFBE|SppA] regulator protein
essential
no
synonyms
yteJ
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.13|Resistance against toxins/ antibiotics]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    3,019,533 3,020,027

    The protein

    Catalyzed reaction/ biological activity

  • binds to and thereby inhibits [protein|ACB7F015027A83C5D3457818A71B6F47192CEFBE|SppA] [pubmed|33028682]

    • [SW|Domains]

  • RDD domain (aa 26-158) (according to UniProt)

    • [SW|Localization]

  • cell membrane [pubmed|33028682]

    • Expression and Regulation

    Operons

    genes
    [gene|ACB7F015027A83C5D3457818A71B6F47192CEFBE|sppA]-[gene|618337D9BCD1586C5874746D1001DA3A2724E57C|sppI]
    description
    [Pubmed|9987136]

    sigma factors

  • [protein|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW]: sigma factor, [Pubmed|9987136,12207695], in [regulon|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW regulon]

    • regulation

  • induced in response to cell wall stress ([protein|search|SigW]) [Pubmed|12207695]

    • additional information

  • self-processes its own C-termini [PubMed|24228759]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-A110 (yteJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/110 NBRP B. subtilis, Japan]
  • BKE29520 ([gene|618337D9BCD1586C5874746D1001DA3A2724E57C|sppI]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TGTCGCGTCCATTATTGTTC, downstream forward: _UP4_TAACCGATAACCCGGACTTC
  • BKK29520 ([gene|618337D9BCD1586C5874746D1001DA3A2724E57C|sppI]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TGTCGCGTCCATTATTGTTC, downstream forward: _UP4_TAACCGATAACCCGGACTTC

    • References

  • 9987136,12207695,31424929,33028682