SubtiBank SubtiBank
nusB [2018-07-20 09:46:55]
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nusB [2018-07-20 09:46:55]

probable transcription termination
locus
BSU24320
pI
4.76
mw
14.74 kDa
protein length
131 aa Sequence Blast
gene length
393 bp Sequence Blast
function
unknown
product
unknown
essential
no
synonyms
yqhZ
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.1|Transcription] → [category|SW 3.2.1.3|Transcription elongation/ termination]

    • Gene

    Coordinates
    2,529,267 → 2,529,662

    The protein

    Protein family

  • nusB family (according to Swiss-Prot)

    • Structure

  • [PDB|1EYV] (from Mycobacterium tuberculosis, 40% identity) [pubmed|10881194]

    • Expression and Regulation

    Operons

    genes
    [gene|6155468AFF216D97373CEF7B8C6E6C7BB9D4617F|nusB]-[gene|71FC6E75694B7066D808BAD736140569875E5E5C|folD]
    description
    [Pubmed|2536750,11591660]

    regulatory mechanism

  • [protein|A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|PurR]: repression, (molecular inducer: PRPP) [Pubmed|2536750,11591660], in [regulon|A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|PurR regulon]
  • [regulon|stringent response|stringent response]: negative regulation, in [regulon|stringent response|stringent response]

    • regulation

  • repressed in the presence of purine nucleotides ([protein|search|PurR]) [Pubmed|2536750,11591660]
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    Biological materials

    Mutant

  • MGNA-C368 (yqhZ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2366 NBRP B. subtilis, Japan]
  • BKE24320 (Δ[gene|6155468AFF216D97373CEF7B8C6E6C7BB9D4617F|nusB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE24320 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTCTTTCTCCTTTGAT, downstream forward: _UP4_TAGGAGGAAAGAAAATGACT
  • BKK24320 (Δ[gene|6155468AFF216D97373CEF7B8C6E6C7BB9D4617F|nusB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK24320 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTCTTTCTCCTTTGAT, downstream forward: _UP4_TAGGAGGAAAGAAAATGACT

    • References

  • 11948165,16707701,11591660,2536750,10881194