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yaaJ [2017-09-27 16:04:45]
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yaaJ [2017-09-27 16:04:45]

tRNA specific adenosine deaminase
locus
BSU00180
pI
5.64
mw
17.61 kDa
protein length
161 aa Sequence Blast
gene length
483 bp Sequence Blast
function
tRNA modification
product
tRNA specific adenosine deaminase
essential
no
synonyms

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.8|tRNA modification and maturation]
  • Gene

    Coordinates
    25,852 → 26,337

    The protein

    Protein family

  • cytidine and deoxycytidylate deaminase family (according to Swiss-Prot)
  • Structure

  • [PDB|2B3J] (the protein of ''Staphylococcus aureus'', complex with RNA) [Pubmed|16415880]
  • Expression and Regulation

    Operons

    genes
    [gene|5FC8D0B30B55C1CBC2CDE8E56E7E948A781EF2AB|yaaJ]-[gene|097C817A5A3E31F73F6ABC4AA95852A64E43A057|scr]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, (both promoters), in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
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    Biological materials

    Mutant

  • MGNA-B891 (yaaJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1890 NBRP B. subtilis, Japan]
  • BKE00180 (Δ[gene|5FC8D0B30B55C1CBC2CDE8E56E7E948A781EF2AB|yaaJ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE00180 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAGGATAAGCTCTCTTT, downstream forward: _UP4_TAGTACGGTTGCAATTTTTA
  • BKK00180 (Δ[gene|5FC8D0B30B55C1CBC2CDE8E56E7E948A781EF2AB|yaaJ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK00180 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAGGATAAGCTCTCTTT, downstream forward: _UP4_TAGTACGGTTGCAATTTTTA
  • References

  • 19087206,12110595,16415880