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ctpA [2019-03-31 16:05:38]
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ctpA [2019-03-31 16:05:38]

carboxy-terminal processing protease, promotes DNA damage checkpoint recovery
locus
BSU19590
pI
8.86
mw
50.98 kDa
protein length
466 aa Sequence Blast
gene length
1401 bp Sequence Blast
function
unknown
product
carboxy-terminal processing protease
essential
no
synonyms
yzbD
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.7|Proteolysis] → [category|SW 3.3.7.4|Additional proteins involved in proteolysis]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    2,131,902 2,133,302

    Phenotypes of a mutant

  • deletion of [protein|5ED8301B9681FDD5171A9344A971E891493A6558|CtpA] and [protein|1A5C1BDE844AA56B90C0E4A02D74978676487E99|DdcP] leads to accumulation of the checkpoint protein [protein|7BF591DCDC9635C605D76135481A8A9DB63EE861|YneA] [pubmed|29979679]
  • sensitivity to DNA damage [pubmed|29979679]

    • The protein

    Catalyzed reaction/ biological activity

  • digestion of [protein|7BF591DCDC9635C605D76135481A8A9DB63EE861|YneA] [pubmed|29979679]

    • Protein family

  • peptidase s41a family (according to Uniprot)

    • Paralogous protein(s)

  • [protein|85247AD09B7A472607B32D57E6318BA6C83EC6BA|CtpB]

    • [SW|Domains]

  • signal peptide (aa 1 - 36) [pubmed|29979679]
  • [SW|PDZ domain] (aa 96- 174) [pubmed|29979679]
  • S41 peptidase domain [pubmed|29979679]
  • peptidoglycan binding domain (C-terminal) [pubmed|29979679]

    • Structure

  • [PDB|4C2D] ([protein|85247AD09B7A472607B32D57E6318BA6C83EC6BA|CtpB], 42% identity) [pubmed|24243021]

    • [SW|Localization]

  • cell membrane, with extracellular protease domain [pubmed|30315724]

    • Expression and Regulation

    Operons

    genes
    [gene|5ED8301B9681FDD5171A9344A971E891493A6558|ctpA]
    description
    [Pubmed|22383849]
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    Biological materials

    Mutant

  • MGNA-A087 (ctpA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/87 NBRP B. subtilis, Japan]
  • 1A866 ( ''ctpA''::''cat''), [Pubmed| ], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A866&Search=1A866 BGSC]
  • BKE19590 ([gene|5ED8301B9681FDD5171A9344A971E891493A6558|ctpA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE19590 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTAAACACCACCTTTTT, downstream forward: _UP4_TAAAAAAAACCATACGCGGC
  • BKK19590 ([gene|5ED8301B9681FDD5171A9344A971E891493A6558|ctpA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK19590 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTAAACACCACCTTTTT, downstream forward: _UP4_TAAAAAAAACCATACGCGGC

    • References

  • 9734814,24243021,29979679,30315724