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rluB [2019-07-09 10:08:31]
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rluB [2019-07-09 10:08:31]

ribosomal large subunit pseudouridine synthase B
locus
BSU23160
pI
10.25
mw
25.88 kDa
protein length
244 aa Sequence Blast
gene length
735 bp Sequence Blast
function
23S rRNA modification
product
pseudouridine synthase
essential
no
ec
5.4.99.22
synonyms
ypuL
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.2|rRNA modification and maturation]

    • Gene

    Coordinates
    2,421,438 2,422,172

    The protein

    Catalyzed reaction/ biological activity

  • uridine2605 in 23S rRNA a pseudouridine2605 in 23S rRNA (according to E. coli RluB)

    • Protein family

  • [SW|S4 RNA-binding domain] superfamily (according to http://www.ebi.ac.uk/interpro/entry/IPR036986 Interpro)
  • pseudouridine synthase rsuA family (together with [protein|69052B24B577ABA4B20E610F679154DC97EFD8DE|YtzG]) (according to Swiss-Prot)

    • [SW|Domains]

  • [SW|S4 RNA-binding domain] (aa 2 ... 68) (according to UniProt)

    • Structure

  • [PDB|4LAB] (from ''E. coli'', 40% identity) [Pubmed|24214967]

    • Expression and Regulation

    Operons

    genes
    [gene|5CD1E266B0EC82546A2AF19A05490DD4D04F812C|rluB]-[gene|F7D869F77E2275110737EF658C58FA1BF742D73F|resA]-[gene|495721E4B8BF6FEC01E62E86339560F90776EED1|resB]-[gene|97FE84CF968596AC39D19EE2E43DA625147FD702|resC]-[gene|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|resD]-[gene|EA6790EF30D3DDB9670FE52DFD2C5083AB6A48E1|resE]
    description
    [Pubmed|22383849]
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    Biological materials

    Mutant

  • MGNA-A106 (ypuL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/106 NBRP B. subtilis, Japan]
  • BKE23160 ([gene|5CD1E266B0EC82546A2AF19A05490DD4D04F812C|rluB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE23160 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCTTTCACCTCAAGTA, downstream forward: _UP4_TAAATTTCACATAACCTTCA
  • BKK23160 ([gene|5CD1E266B0EC82546A2AF19A05490DD4D04F812C|rluB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK23160 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCTTTCACCTCAAGTA, downstream forward: _UP4_TAAATTTCACATAACCTTCA

    • References

  • 9888802,24214967