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kimA [2017-05-09 15:36:35]
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kimA [2017-05-09 15:36:35]

high-affinity potassium transporter (uptake)
locus
BSU04320
pI
9.93
mw
66.60 kDa
protein length
607 aa Sequence Blast
gene length
1821 bp Sequence Blast
function
potassium uptake
product
high-affinity potassium transporter
essential
no
synonyms
ydaO
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.5|Metal ion transporter]
  • [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.1|Metal ion homeostasis (K, Na, Ca, Mg)] → [category|SW 1.3.1.3|Potassium uptake/ export]
  • [category|SW 3|Information processing] → [category|SW 3.5|Targets of second messengers] → [category|SW 3.5.1|Targets of c-di-AMP]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    486,432 → 488,255

    Phenotypes of a mutant

  • a [gene|search|kimA ][gene|search|ktrA ][gene|search|ktrB ]mutant requires high potassium concentrations on minimal medium with ammonium as nitrogen source [pubmed|28420751]

    • The protein

    Catalyzed reaction/ biological activity

  • uptake of potassium [pubmed|28420751]

    • [SW|Domains]

  • 11 trans-membrane helices at the N-terminus [pubmed|28420751]

    • [SW|Localization]

  • cell membrane (according to [http://www.uniprot.org/uniprot/P96589 UniProt])

    • Expression and Regulation

    Operons

    genes
    [gene|5968E6D4D7378C69EC3E1E05B285069123AA1FD3|kimA]
    description
    [Pubmed|22383849]

    regulatory mechanism

  • [regulon|ydaO riboswitch|ydaO riboswitch]: termination/antitermination, expression is switched off upon binding of c-di-AMP (formed at high potassium concentration) [pubmed|28420751], in [regulon|ydaO riboswitch|ydaO riboswitch]

    • regulation

  • expressed at low potassium concentration ([regulon|ydaO riboswitch|ydaO riboswitch]) [Pubmed|28420751]
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    Biological materials

    Mutant

  • MGNA-C090 (kimA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2088 NBRP B. subtilis, Japan]
  • 1A688 (''[gene|5968E6D4D7378C69EC3E1E05B285069123AA1FD3|kimA]''::''erm''), available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A688&Search=1A688 BGSC]
  • GP93 (''[gene|5968E6D4D7378C69EC3E1E05B285069123AA1FD3|kimA]''::''cat''), available in [SW|Jörg Stülke]'s lab [pubmed|28420751]

    • Expression vector

  • pGP2913 (N-terminal His-tag, expression in ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

    • lacZ fusion

  • BP144 (based on [SW|pAC5]), available in [SW|Fabian Commichau]' and [SW|Jörg Stülke]'s labs [pubmed|28420751]

    • FLAG-tag construct

  • GP2405 ''ydaO-3xFLAG spc'' (based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab [pubmed|28420751]

    • References

    Reviews

  • 25869574

    • Original publications

  • 15096624,20511502,23086297,24141192,25086509,25086507,28420751