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rnpA [2019-04-24 10:15:18]
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rnpA [2019-04-24 10:15:18]

protein component of RNase P, metal-dependent 5 end maturation of precursor tRNAs
locus
BSU41050
pI
10.80
mw
13.66 kDa
protein length
116 aa Sequence Blast
gene length
351 bp Sequence Blast
function
cleavage of precursor sequences from the 5 ends of pre-tRNAs
product
protein component of RNase P (substrate specificity)
essential
yes
ec
3.1.26.5
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.4|RNases] → [category|SW 3.2.4.2|Endoribonucleases]
  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.8|tRNA modification and maturation]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

    • Gene

    Coordinates
    4,214,753 4,215,103

    Phenotypes of a mutant

  • essential [Pubmed|12682299]

    • The protein

    Catalyzed reaction/ biological activity

  • Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from [gene|3B6620F44EC08BA9B02FE9359A5F74C6CAE6D4FB|trnA-Ile] precursor (according to Swiss-Prot)
  • 5' end maturation of tmRNA [Pubmed|25402410]

    • Protein family

  • rnpA family (according to Swiss-Prot)

    • [SW|Domains]

  • RNR motif: residues 60-68, stabilizes complexes with both RNA part of [SW|RNase] P (''[gene|57A79AAF00243B5E058FA901D43AA7B55CA33F63|rnpB]'') and with pre-[gene|3B6620F44EC08BA9B02FE9359A5F74C6CAE6D4FB|trnA-Ile] ([gene|57A79AAF00243B5E058FA901D43AA7B55CA33F63|rnpB]-[protein|594F2D36BE71A45303A33AA2A2931F56F0A969ED|RnpA]-pre-[gene|3B6620F44EC08BA9B02FE9359A5F74C6CAE6D4FB|trnA-Ile]) [Pubmed|21622899]

    • Structure

  • [PDB|1A6F] [Pubmed|9563955]
  • [PDB|3Q1R] (complex of RnpA with RNA [protein|57A79AAF00243B5E058FA901D43AA7B55CA33F63|RnpB] and [gene|3B6620F44EC08BA9B02FE9359A5F74C6CAE6D4FB|trnA-Ile], from ''Thermotoga maritima'') [Pubmed|21076397]

    • Expression and Regulation

    Operons

    genes
    [gene|EB07AECD3849CC0B65126DE27E71B28B3AD106B0|rpmH]-[gene|594F2D36BE71A45303A33AA2A2931F56F0A969ED|rnpA]-[gene|D40C202E67A5319A91811DB11356F47A56C97DD2|yidC1]-[gene|23D33E2D7AB1CA02165070DB8238957E3C768E64|jag]
    description
    [Pubmed|22383849]
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    labs

  • [SW|Carol Fierke], University of Michigan, Ann Arbor, USA [https://www.chem.lsa.umich.edu/chem/faculty/facultyDetail.php?Uniqname=fierke homepage]
  • [SW|Roland Hartmann], Marburg University, Germany [http://www.pharmazie.uni-marburg.de/pharmchem/akhartmann/ag_hartmann.htm homepage]

    • References

    Reviews

  • 19215776,8901495,17868095,14691942,16650980,16980936,16679018,14550630

    • Original Publications

  • 10390342,17919279,17355991,11812129,16980484,11454206,19549719,11454206,9563955,19932118,17299131,20476778,11258888,16185070,21764917,22848659,21076397,20843005,21622899,24364358,25249623,25402410,26267651,27873254